ArticleMolecular immunology
Dynamic DNA methylation patterns across the mouse and human IL10 genes during CD4+ T cell activation; influence of IL-27.
Article in Molecular immunology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Analysis of IL10 gene promoter haplotypes and changes in mRNA expression and soluble levels in patients with basal cell carcinoma.Archives of dermatological research · 2025Article
- The Impact of the IL-10 Gene Polymorphism on mRNA Expression and IL-10 Serum Concentration in Polish Lupus Patients.International journal of molecular sciences · 2024Article
- Mother's smoking habits affects IL10 methylation but not asthma in Ecuadorian children.Molecular genetics & genomic medicine · 2024Article
- Interleukin-27 Functional Duality BalancesFrontiers in immunology · 2020Review
- Paternal Tobacco Smoke Correlated to Offspring Asthma and Prenatal Epigenetic Programming.Frontiers in genetics · 2019Article
- The molecular pathophysiology of chronic non-bacterial osteomyelitis (CNO)-a systematic review.Molecular and cellular pediatrics · 2017Review
- Epigenetics in SLE.Current rheumatology reports · 2017Review
- DNA methylation in systemic lupus erythematosus.Epigenomics · 2017Review
- Human interleukin-27: wide individual variation in plasma levels and complex inter-relationships with interleukin-17A.Clinical and experimental immunology · 2014Article
- Stat3 promotes IL-10 expression in lupus T cells through trans-activation and chromatin remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- A protective role for human IL-10-expressing CD4+ T cells in colitis.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Pilot study: possible association of IL10 promoter polymorphisms with CRMO.Rheumatology international · 2012Article
- IL-10-producing Th1 cells and disease progression are regulated by distinct CD11c⁺ cell populations during visceral leishmaniasis.PLoS pathogens · 2012Article
- Update: Cytokine Dysregulation in Chronic Nonbacterial Osteomyelitis (CNO).International journal of rheumatology · 2012Article
- cAMP-responsive element modulator (CREM)α protein signaling mediates epigenetic remodeling of the human interleukin-2 gene: implications in systemic lupus erythematosus.The Journal of biological chemistry · 2011Article
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Authors and funding
6 authors at 1 institution in 1 country.
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Abstract
IL-10 plays a critical role in controlling inflammation and the anti-inflammatory functions of IL-10 are regulated based on its coordinated expression from various cellular sources, most notably T cells. Although nearly all CD4+ subpopulations can express IL-10, surprisingly little is known about the molecular mechanisms which control IL-10 induction, particularly in humans. To examine the regulation of human IL-10 expression, we created the hIL10BAC transgenic mouse. As previously reported, we observed conservation of myeloid-derived IL-10 expression but found that human IL-10 was only weakly expressed in splenic CD4+ T cells from hIL10BAC mice. Since DNA methylation is an important determinant of gene expression profiles, we assessed the patterns of DNA methylation in the human and mouse IL10 genes in naïve and activated CD4+ T cells. Across mouse and human IL10 there were no obvious patterns of CpG methylation in naïve CD4+ T cells following polyclonal activation. Overall however, the human IL10 gene had significantly higher levels of DNA methylation. Interestingly, coculture with the IL-10-inducing cytokine IL-27 lead to a site-specific reduction in methylation of the mouse but not human IL10 gene. Demethylation was specifically localized to an intronic site adjacent to a known regulatory region. Our findings indicate that while the mouse and human IL10 genes undergo variable changes in DNA methylation during CD4+ T cell activation, IL-27 appears to influence DNA methylation in a particular intronic region thus associating with IL-10 expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.