ArticleJournal of chromatography. A2010
Streamlined pentafluorophenylpropyl column liquid chromatography-tandem quadrupole mass spectrometry and global (13)C-labeled internal standards improve performance for quantitative metabolomics in bacteria.
Article in Journal of chromatography. A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 76 citations in OpenAlex.
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- Statistics and Machine Learning in Mass Spectrometry-Based Metabolomics Analysis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Methylotroph Quorum Sensing Signal Identification by Inverse Stable Isotopic Labeling.ACS chemical biology · 2021Article
- Analytical Platforms for Mass Spectrometry-Based Metabolomics of Polar and Ionizable Metabolites.Advances in experimental medicine and biology · 2021Article
- Rumen Microbiome and Metabolome of Tibetan Sheep (Frontiers in veterinary science · 2020Article
- Systematic Error Removal Using Random Forest for Normalizing Large-Scale Untargeted Lipidomics Data.Analytical chemistry · 2019Article
- Mass Spectrometry in Advancement of Redox Precision Medicine.Advances in experimental medicine and biology · 2019Review
- Extraction and Quantitation of Nicotinamide Adenine Dinucleotide Redox Cofactors.Antioxidants & redox signaling · 2018Article
- Metabolomics Investigation of an Association of Induced Features and Corresponding Fungus during the Co-culture ofFrontiers in microbiology · 2017Article
- Comprehensive molecular characterization of Methylobacterium extorquens AM1 adapted for 1-butanol tolerance.Biotechnology for biofuels · 2016Article
- Article
- Avoiding misannotation of in-source fragmentation products as cellular metabolites in liquid chromatography-mass spectrometry-based metabolomics.Analytical chemistry · 2015Article
- Validation of a dual LC-HRMS platform for clinical metabolic diagnosis in serum, bridging quantitative analysis and untargeted metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2014Article
- Review
- Analysis of selected sugars and sugar phosphates in mouse heart tissue by reductive amination and liquid chromatography-electrospray ionization mass spectrometry.Analytical chemistry · 2013Article
- Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part II. Metabolomics and 13C-Labeling Study.Frontiers in microbiology · 2013Article
- Cardiac-specific deletion of acetyl CoA carboxylase 2 prevents metabolic remodeling during pressure-overload hypertrophy.Circulation research · 2012Article
- Roles of glucose in photoreceptor survival.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Streamlined quantitative metabolomics in central metabolism of bacteria would be greatly facilitated by a high-efficiency liquid chromatography (LC) method in conjunction with accurate quantitation. To achieve this goal, a methodology for LC-tandem quadrupole mass spectrometry (LC-MS/MS) involving a pentafluorophenylpropyl (PFPP) column and culture-derived global (13)C-labeled internal standards (I.Ss.) has been developed and compared to hydrophilic interaction liquid chromatography (HILIC)-MS/MS and published combined two-dimensional gas chromatography and LC methods. All 50 tested metabolite standards from 5 classes (amino acids, carboxylic acids, nucleotides, acyl-CoAs and sugar phosphates) displayed good chromatographic separation and sensitivity on the PFPP column. In addition, many important critical pairs such as isomers/isobars (e.g. isoleucine/leucine, methylsuccinic acid/ethylmalonic acid and malonyl-CoA/3-hydroxybutyryl-CoA) and metabolites of similar structure (e.g. malate/fumarate) were resolved better on the PFPP than on the HILIC column. Compared to only one (13)C-labeled I.S., the addition of global (13)C-labeled I.Ss. improved quantitative linearity and accuracy. PFPP-MS/MS with global (13)C-labeled I.Ss. allowed the absolute quantitation of 42 metabolite pool sizes in Methylobacterium extorquens AM1. A comparison of metabolite level changes published previously for ethylamine (C2) versus succinate (C4) cultures of M. extorquens AM1 indicated a good consistency with the data obtained by PFPP-MS/MS, suggesting this single approach has the capability of providing comprehensive metabolite profiling similar to the combination of methods. The more accurate quantification obtained by this method forms a fundamental basis for flux measurements and can be used for metabolism modeling in bacteria in future studies.
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