ArticleIntegrative biology : quantitative biosciences from nano to macro2010
Open access microfluidic device for the study of cell migration during chemotaxis.
Article in Integrative biology : quantitative biosciences from nano to macro, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Dynamic cluster field modeling of collective chemotaxis.Scientific reports · 2024Article
- A Bi-Directional Acoustic Micropump Driven by Oscillating Sharp-Edge Structures.Micromachines · 2023Article
- A Concentration Gradients Tunable Generator with Adjustable Position of the Acoustically Oscillating Bubbles.Micromachines · 2020Article
- An open-chamber flow-focusing device for focal stimulation of micropatterned cells.Biomicrofluidics · 2016Article
- Cell Blebbing in Confined Microfluidic Environments.PloS one · 2016Article
- Design of a microfluidic device to quantify dynamic intra-nuclear deformation during cell migration through confining environments.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures.Lab on a chip · 2015Article
- A microfluidic technique to probe cell deformability.Journal of visualized experiments : JoVE · 2014Article
- Review
- A microfluidic-enabled mechanical microcompressor for the immobilization of live single- and multi-cellular specimens.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2014Article
- Geometry-Driven Polarity in Motile Amoeboid Cells.PloS one · 2014Article
- Delineating the core regulatory elements crucial for directed cell migration by examining folic-acid-mediated responses.Journal of cell science · 2013Article
- On-chip open microfluidic devices for chemotaxis studies.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012Article
- Femtosecond laser machined microfluidic devices for imaging of cells during chemotaxis.Journal of laser applications · 2011Article
- A modular cell culture device for generating arrays of gradients using stacked microfluidic flows.Biomicrofluidics · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cells sense and interpret chemical gradients, and respond by localized responses that lead to directed migration. An open microfluidic device (OMD) was developed to provide quantitative information on both the gradient and morphological changes that occurred as cells crawled through various microfabricated channels. This device overcame problems that many current devices have been plagued with, such as complicated cell loading, media evaporation and channel blockage by air bubbles. We used a micropipette to set up stable gradients formed by passive diffusion and thus avoided confounding cellular responses produced by shear forces. Two versions of the OMD are reported here: one device that has channels with widths of 6, 8, 10 and 12 μm, while the other has two large 100 μm channels to minimize cellular interaction with lateral walls. These experiments compared the migration rates and qualitative behavior of Dictyostelium discoideum cells responding to measurable cAMP and folic acid gradients in small and large channels. We report on the influence that polarity has on a cell's ability to migrate when confined in a channel. Polarized cells that migrated to cAMP were significantly faster than the unpolarized cells that crawled toward folic acid. Unpolarized cells in wide channels often strayed off course, yet migrated faster than unpolarized cells in confined channels. Cells in channels farthest from the micropipette migrated through the channels at rates similar to cells in channels with higher concentrations, suggesting that cell speed was independent of mean concentration. Lastly, it was found that the polarized cells could easily change migration direction even when only the leading edge of the cell was exposed to a lateral gradient.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.