ArticlePloS one2010
Characterizing DNA condensation and conformational changes in organic solvents.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Gold Nanosystems Covered with Doxorubicin/DNA Complexes: A Therapeutic Target for Prostate and Liver Cancer.International journal of molecular sciences · 2022Article
- Block Copolymer Micellization of DNA Origami Promotes Solubility in Organic Solvents.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Towards DNA-Encoded Micellar Chemistry: DNA-Micelle Association and Environment Sensitivity of Catalysis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Modular self-assembly of gamma-modified peptide nucleic acids in organic solvent mixtures.Nature communications · 2020Article
- Fabrication and characterization of PLGA nanoparticles encapsulating large CRISPR-Cas9 plasmid.Journal of nanobiotechnology · 2020Article
- Synthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains.Journal of fluorescence · 2016Article
- The structural stability and catalytic activity of DNA and RNA oligonucleotides in the presence of organic solvents.Biophysical reviews · 2016Review
- Article
- Structure and properties of DNA in apolar solvents.The journal of physical chemistry. B · 2014Article
- Ion competition in condensed DNA arrays in the attractive regime.Biophysical journal · 2013Article
- Plasmid-templated shape control of condensed DNA-block copolymer nanoparticles.Advanced materials (Deerfield Beach, Fla.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organic solvents offer a new approach to formulate DNA into novel structures suitable for gene delivery. In this study, we examined the in situ behavior of DNA in N, N-dimethylformamide (DMF) at low concentration via laser light scattering (LLS), TEM, UV absorbance and Zeta potential analysis. Results revealed that, in DMF, a 21bp oligonucleotide remained intact, while calf thymus DNA and supercoiled plasmid DNA were condensed and denatured. During condensation and denaturation, the size was decreased by a factor of 8-10, with calf thymus DNA forming spherical globules while plasmid DNA exhibited a toroid-like conformation. In the condensed state, DNA molecules were still able to release the counterions to be negatively charged, indicating that the condensation was mainly driven by the excluded volume interactions. The condensation induced by DMF was reversible for plasmid DNA but not for calf thymus DNA. When plasmid DNA was removed from DMF and resuspended in an aqueous solution, the DNA was quickly regained a double stranded configuration. These findings provide further insight into the behavior and condensation mechanism of DNA in an organic solvent and may aid in developing more efficient non-viral gene delivery systems.
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