ArticlePloS one2010
A genetic screen for attenuated growth identifies genes crucial for intraerythrocytic development of Plasmodium falciparum.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- A luciferase-based hRBC-NSG mouse model to evaluate anti-CelTOS transmission-blocking antibodies against P. falciparum.Malaria journal · 2026Article
- Experimental vaccination by single dose sporozoite injection of blood-stage attenuated malaria parasites.EMBO molecular medicine · 2024Article
- Multistage protective anti-CelTOS monoclonal antibodies with cross-species sterile protection against malaria.Nature communications · 2024Article
- A novel Modulator of Ring Stage Translation (MRST) gene alters artemisinin sensitivity inmSphere · 2023Article
- Phenotypic Screens Identify Genetic Factors Associated with Gametocyte Development in the Human Malaria Parasite Plasmodium falciparum.Microbiology spectrum · 2023Article
- CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact.Biochemical Society transactions · 2022Review
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- AttenuatedFrontiers in immunology · 2020Article
- ApiAP2 Transcription Factors in Apicomplexan Parasites.Pathogens (Basel, Switzerland) · 2019Review
- Plasmodium male gametocyte development and transmission are critically regulated by the two putative deadenylases of the CAF1/CCR4/NOT complex.PLoS pathogens · 2019Article
- Integrated pathogen load and dual transcriptome analysis of systemic host-pathogen interactions in severe malaria.Science translational medicine · 2018Article
- Comparative Heterochromatin Profiling Reveals Conserved and Unique Epigenome Signatures Linked to Adaptation and Development of Malaria Parasites.Cell host & microbe · 2018Article
- The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum.Genome biology · 2016Article
- Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants.Genome research · 2016Article
- Advances in molecular genetic systems in malaria.Nature reviews. Microbiology · 2015Review
- Artemisinin-resistant Plasmodium falciparum parasites exhibit altered patterns of development in infected erythrocytes.Antimicrobial agents and chemotherapy · 2015Article
- In silico characterization of an atypical MAPK phosphatase of Plasmodium falciparum as a suitable target for drug discovery.Chemical biology & drug design · 2014Article
- Identifying novel cell cycle proteins in Apicomplexa parasites through co-expression decision analysis.PloS one · 2014Article
- Atypical mitogen-activated protein kinase phosphatase implicated in regulating transition from pre-S-Phase asexual intraerythrocytic development of Plasmodium falciparum.Eukaryotic cell · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A majority of the Plasmodium falciparum genome codes for genes with unknown functions, which presents a major challenge to understanding the parasite's biology. Large-scale functional analysis of the parasite genome is essential to pave the way for novel therapeutic intervention strategies against the disease and yet difficulties in genetic manipulation of this deadly human malaria parasite have been a major hindrance for functional analysis of its genome. Here, we used a forward functional genomic approach to study P. falciparum and identify genes important for optimal parasite development in the disease-causing, intraerythrocytic stages. We analyzed 123 piggyBac insertion mutants of P. falciparum for proliferation efficiency in the intraerythrocytic stages, in vitro. Almost 50% of the analyzed mutants showed significant reduction in proliferation efficiency, with 20% displaying severe defects. Functional categorization of genes in the severely attenuated mutants revealed significant enrichment for RNA binding proteins, suggesting the significance of post-transcriptional gene regulation in parasite development and emphasizing its importance as an antimalarial target. This study demonstrates the feasibility of much needed forward genetics approaches for P. falciparum to better characterize its genome and accelerate drug and vaccine development.
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