ArticlePloS one2010
Near-membrane dynamics and capture of TRPM8 channels within transient confinement domains.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Pharmacological tools to modulate ordered membrane domains and order-dependent protein function.Communications chemistry · 2026Article
- Pharmacological Tools to Modulate Ordered Membrane Domains and Order-Dependent Protein Function.bioRxiv : the preprint server for biology · 2025Article
- Small-Molecule Modulators of Lipid Raft Stability and Protein-Raft Partitioning.bioRxiv : the preprint server for biology · 2024Article
- Residues of TRPM8 at the Lipid-Water-Interface have Coevolved with Cholesterol Interaction and are Relevant for Diverse Health Disorders.The Journal of membrane biology · 2024Article
- The odyssey of the TR(i)P journey to the cellular membrane.Frontiers in cell and developmental biology · 2024Review
- Counter-Intuitive Features of Particle Dynamics in Nanopores.International journal of molecular sciences · 2023Review
- Regulation of ThermoTRP Channels by PIP2 and Cholesterol.Advances in experimental medicine and biology · 2023Article
- Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression.International journal of molecular sciences · 2022Article
- Regulation of thermoTRPs by lipids.Temperature (Austin, Tex.) · 2017Review
- Journey of a cold sensor - VAMP7-dependent transport of TRPM8.Channels (Austin, Tex.) · 2016Article
- VAMP7 regulates constitutive membrane incorporation of the cold-activated channel TRPM8.Nature communications · 2016Article
- Aged insulin granules display reduced microtubule-dependent mobility and are disposed within actin-positive multigranular bodies.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Agonist-dependent modulation of cell surface expression of the cold receptor TRPM8.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity.The Journal of cell biology · 2015Article
- Distinct modes of perimembrane TRP channel turnover revealed by TIR-FRAP.Scientific reports · 2014Article
- Motor and tail homology 1 (Th1) domains antagonistically control myosin-1 dynamics.Biophysical journal · 2014Article
- A structural view of ligand-dependent activation in thermoTRP channels.Frontiers in physiology · 2014Review
- A failure in energy metabolism and antioxidant uptake precede symptoms of Huntington's disease in mice.Nature communications · 2013Article
- Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana.Plant methods · 2011Article
- Modulating the surface expression of cold receptors.Temperature (Austin, Tex.)Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cold and menthol receptor, TRPM8, is a non-selective cation channel expressed in a subset of peripheral neurons that is responsible for neuronal detection of environmental cold stimuli. It was previously shown that members of the transient receptor potential (TRP) family of ion channels are translocated toward the plasma membrane (PM) in response to agonist stimulation. Because the spatial and temporal dynamics of cold receptor cell-surface residence may determine neuronal activity, we hypothesized that the movement of TRPM8 to and from the PM might be a regulated process. Single particle tracking (SPT) is a useful tool for probing the organization and dynamics of protein constituents in the plasma membrane. METHODOLOGY/PRINCIPAL
findingsWe used SPT to study the receptor dynamics and describe membrane/near-membrane behavior of particles containing TRPM8-EGFP in transfected HEK-293T and F-11 cells. Cells were imaged using total internal reflection fluorescence (TIRF) microscopy and the 2D and 3D trajectories of TRPM8 molecules were calculated by analyzing mean-square particle displacement against time. Four characteristic types of motion were observed: stationary mode, simple Brownian diffusion, directed motion, and confined diffusion. In the absence of cold or menthol to activate the channel, most TRPM8 particles move in network covering the PM, periodically lingering for 2-8 s in confined microdomains of about 800 nm radius. Removing cholesterol with methyl-beta-cyclodextrin (MβCD) stabilizes TRPM8 motion in the PM and is correlated with larger TRPM8 current amplitude that results from an increase in the number of available channels without a change in open probability. CONCLUSIONS/SIGNIFICANCE: These results reveal a novel mechanism for regulating TRPM8 channel activity, and suggest that PM dynamics may play an important role in controlling electrical activity in cold-sensitive neurons.
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