ArticleProceedings of the National Academy of Sciences of the United States of America2010
Photooxidation of cytochrome P450-BM3.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 100 citations in OpenAlex.
- Oxidizable amino acids around cytochrome P450 hemes.Journal of inorganic biochemistry · 2026Article
- Expanding Perchlorate Use for C-H Oxidative Transformations: A Tandem Photo- and Iron-Catalytic Strategy.Organometallics · 2025Article
- The Impact of Inorganic Systems and Photoactive Metal Compounds on Cytochrome P450 Enzymes and Metabolism: From Induction to Inhibition.Biomolecules · 2024Review
- Photocatalytic generation of a non-heme Fe(iii)-hydroperoxo species with OChemical science · 2022Article
- Oxygenating Biocatalysts for Hydroxyl Functionalisation in Drug Discovery and Development.ChemMedChem · 2022Review
- Controlling the optical and catalytic properties of artificial metalloenzyme photocatalysts using chemogenetic engineering.Chemical science · 2022Article
- Ru(II)-diimine complexes and cytochrome P450 working hand-in-hand.Journal of inorganic biochemistry · 2020Review
- Biotransformation of Ethinylestradiol by Whole Cells of Brazilian Marine-Derived Fungus Penicillium oxalicum CBMAI 1996.Marine biotechnology (New York, N.Y.) · 2020Article
- Light-driven catalysis with engineered enzymes and biomimetic systems.Biotechnology and applied biochemistry · 2020Review
- Electron Transfer in Nitrogenase.Chemical reviews · 2020Review
- Hole Hopping through Cytochrome P450.The journal of physical chemistry. B · 2020Article
- Recent developments in enantioselective photocatalysis.Beilstein journal of organic chemistry · 2020Review
- Under the spotlight: mechanisms of photobiomodulation concentrating on blue and green light.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2019Article
- Living with Oxygen.Accounts of chemical research · 2018Article
- A photoactive semisynthetic metalloenzyme exhibits complete selectivity for COChemical communications (Cambridge, England) · 2018Article
- Keeping the spotlight on cytochrome P450.Biochimica et biophysica acta. Proteins and proteomics · 2018Review
- Hole Hopping through Tryptophan in Cytochrome P450.Biochemistry · 2017Article
- Insights into an efficient light-driven hybrid P450 BM3 enzyme from crystallographic, spectroscopic and biochemical studies.Biochimica et biophysica acta · 2016Article
- Design and engineering of artificial oxygen-activating metalloenzymes.Chemical Society reviews · 2016Review
- The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases?Chembiochem : a European journal of chemical biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
High-valent iron-oxo species are thought to be intermediates in the catalytic cycles of oxygenases and peroxidases. An attractive route to these iron-oxo intermediates involves laser flash-quench oxidation of ferric hemes, as demonstrated by our work on the ferryl (compound II) and ferryl porphyrin radical cation (compound I) intermediates of horseradish peroxidase. Extension of this work to include cytochrome P450-BM3 (CYP102A1) has required covalent attachment of a Ru(II) photosensitizer to a nonnative cysteine near the heme (RuIIK97C-FeIIIP450), in order to promote electron transfer from the Fe(III) porphyrin to photogenerated Ru(III). The conjugate was structurally characterized by X-ray crystallography (2.4 Å resolution; Ru-Fe distance, 24 Å). Flash-quench oxidation of the ferric-aquo heme produces an Fe(IV)-hydroxide species (compound II) within 2 ms. Difference spectra for three singly oxidized P450-BM3 intermediates were obtained from kinetics modeling of the transient absorption data in combination with generalized singular value decomposition analysis and multiexponential fitting.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.