ArticleThe Journal of biological chemistry2010
Novel channel enzyme fusion proteins confer arsenate resistance.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Synergistic function of ars genes in arsenic detoxification and biosynthesis of organoarsenicals.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Review
- Intrinsic Asymmetry in Weak Acid Transmembrane Transporters.Biomolecules · 2026Review
- Local Attraction of Substrates and Co-Substrates Enhances Weak Acid and Base Transmembrane Transport.Biomolecules · 2022Review
- Is Arsenic Exposure a Risk Factor for Metabolic Syndrome? A Review of the Potential Mechanisms.Frontiers in endocrinology · 2022Review
- Identification and Genome Analysis of an Arsenic-Metabolizing Strain ofBioMed research international · 2022Article
- Mechanism for Higher Tolerance to and Lower Accumulation of Arsenite inPlants (Basel, Switzerland) · 2020Article
- Transmembrane Facilitation of Lactate/HMembranes · 2020Article
- Formate-nitrite transporters carrying nonprotonatable amide amino acids instead of a central histidine maintain pH-dependent transport.The Journal of biological chemistry · 2019Article
- Identification of Resistance Genes and Response to Arsenic inFrontiers in microbiology · 2019Article
- Distribution of Arsenic Resistance Genes in Prokaryotes.Frontiers in microbiology · 2018Review
- Targeting Channels and Transporters in Protozoan Parasite Infections.Frontiers in chemistry · 2018Review
- Pentamidine Is Not a Permeant but a Nanomolar Inhibitor of the Trypanosoma brucei Aquaglyceroporin-2.PLoS pathogens · 2016Article
- The ins and outs of metal homeostasis by the root nodule actinobacterium Frankia.BMC genomics · 2014Article
- Genome-wide association mapping identifies a new arsenate reductase enzyme critical for limiting arsenic accumulation in plants.PLoS biology · 2014Article
- Aquaglyceroporins: generalized metalloid channels.Biochimica et biophysica acta · 2014Review
- Aquaporins with anion/monocarboxylate permeability: mechanisms, relevance for pathogen-host interactions.Frontiers in pharmacology · 2014Review
- Use of whole genome sequencing to determine the microevolution of Mycobacterium tuberculosis during an outbreak.PloS one · 2013Article
- Functional characterization of a novel aquaporin from Dictyostelium discoideum amoebae implies a unique gating mechanism.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Steady exposure to environmental arsenic has led to the evolution of vital cellular detoxification mechanisms. Under aerobic conditions, a two-step process appears most common among microorganisms involving reduction of predominant, oxidized arsenate (H(2)As(V)O(4)(-)/HAs(V)O(4)(2-)) to arsenite (As(III)(OH)(3)) by a cytosolic enzyme (ArsC; Escherichia coli type arsenate reductase) and subsequent extrusion via ArsB (E. coli type arsenite transporter)/ACR3 (yeast type arsenite transporter). Here, we describe novel fusion proteins consisting of an aquaglyceroporin-derived arsenite channel with a C-terminal arsenate reductase domain of phosphotyrosine-phosphatase origin, providing transposable, single gene-encoded arsenate resistance. The fusion occurred in actinobacteria from soil, Frankia alni, and marine environments, Salinispora tropica; Mycobacterium tuberculosis encodes an analogous ACR3-ArsC fusion. Mutations rendered the aquaglyceroporin channel more polar resulting in lower glycerol permeability and enhanced arsenite selectivity. The arsenate reductase domain couples to thioredoxin and can complement arsenate-sensitive yeast strains. A second isoform with a nonfunctional channel may use the mycothiol/mycoredoxin cofactor pool. These channel enzymes constitute prototypes of a novel concept in metabolism in which a substrate is generated and compartmentalized by the same molecule. Immediate diffusion maintains the dynamic equilibrium and prevents toxic accumulation of metabolites in an energy-saving fashion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.