ArticleJournal of the American Society for Mass Spectrometry2010
The extent and effects of peptide sequence scrambling via formation of macrocyclic B ions in model proteins.
Article in Journal of the American Society for Mass Spectrometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Implication ofInternational journal of molecular sciences · 2025Article
- The role of the mobile proton in fucose migration.Analytical and bioanalytical chemistry · 2019Article
- DNA Oligonucleotide Fragment Ion Rearrangements Upon Collision-Induced Dissociation.Journal of the American Society for Mass Spectrometry · 2015Article
- Peptide scrambling during collision-induced dissociation is influenced by N-terminal residue basicity.Journal of the American Society for Mass Spectrometry · 2014Article
- The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics.Nature chemistry · 2014Review
- Gas-phase ion isomer analysis reveals the mechanism of peptide sequence scrambling.Analytical chemistry · 2014Article
- Structures of a(n)* ions derived from protonated pentaglycine and pentaalanine: results from IRMPD spectroscopy and DFT calculations.Journal of the American Society for Mass Spectrometry · 2013Article
- Evidence for sequence scrambling in collision-induced dissociation of y-type fragment ions.Journal of the American Society for Mass Spectrometry · 2013Article
- Fragmentation reactions of methionine-containing protonated octapeptides and fragment ions therefrom: an energy-resolved study.Journal of the American Society for Mass Spectrometry · 2013Article
- Non-direct sequence ions in the tandem mass spectrometry of protonated peptide amides--an energy-resolved study.Journal of the American Society for Mass Spectrometry · 2013Article
- Investigation of scrambled ions in tandem mass spectra, part 2. On the influence of the ions on peptide identification.Journal of the American Society for Mass Spectrometry · 2013Article
- Selective deletion of the internal lysine residue from the peptide sequence by collisional activation.Journal of the American Society for Mass Spectrometry · 2012Article
- Top-down study of β2-microglobulin deamidation.Analytical chemistry · 2012Article
- Investigation of scrambled ions in tandem mass spectra. Part 1. Statistical characterization.Journal of the American Society for Mass Spectrometry · 2012Article
- Conformation-specific spectroscopy of peptide fragment ions in a low-temperature ion trap.Journal of the American Society for Mass Spectrometry · 2012Article
- Fragmentation reactions of b(5) and a (5) ions containing proline--the structures of a(5) ions.Journal of the American Society for Mass Spectrometry · 2012Article
- Disfavoring macrocycle b fragments by constraining torsional freedom: the "twisted" case of QWFGLM b6.Journal of the American Society for Mass Spectrometry · 2012Article
- Occurrence of C-terminal residue exclusion in peptide fragmentation by ESI and MALDI tandem mass spectrometry.Journal of the American Society for Mass Spectrometry · 2012Article
- Mass spectrometry analysis of 2-nitrophenylhydrazine carboxy derivatized peptides.Journal of the American Society for Mass Spectrometry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extent and effects of sequence scrambling in peptide ions during tandem mass spectrometry (MS/MS) have been examined using tryptic peptides from model proteins. Sequence-scrambled b ions appeared in about 35% of 43 tryptic peptides examined under MS/MS conditions. In general, these ions had relatively low abundances with averages of 8% and 16%, depending on the instrumentation used. A few tryptic peptides gave abundant scrambled b ions in MS/MS. However, peptide and protein identifications under proteomic conditions with Mascot were not affected, even for these peptides wherein scrambling was prominent. From the 43 tryptic peptides that have been investigated, the conclusion is that sequence scrambling is unlikely to impact negatively on the accuracy of automated peptide and protein identifications in proteomics.
Indexed as
Identifiers
20947370What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.