Evidence map›Paper›PMID 20946790›Full record

ArticleMethods in enzymology2009

Analysis of RNA folding by native polyacrylamide gel electrophoresis.

Sarah A Woodson, Eda Koculi

Open access · greenAbstract read
In one paragraph

Article in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 61 citations in OpenAlex.

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  12. CNature communications · 2024
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  16. Adoption of A-Z Junctions in RNAs by Binding of Zα Domains.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
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  20. Structural basis for impaired 5' processing of a mutant tRNA associated with defects in neuronal homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Sarah A WoodsonT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA.
Eda Koculi
Johns Hopkins University · USNorthwestern University · US

Funding

Role of Exon Structure in Splicing of Group I IntronsR01GM046686 · NIGMS · UNIVERSITY OF MARYLAND COLLEGE PK CAMPUS · PI WOODSON, SARAH A. · 1992 to 2014
$4.0M
NIGMS NIH HHS GM46686NIGMS NIH HHS R01 GM046686
6 · The paper itself

Abstract

Polyacrylamide gel electrophoresis under native conditions (native PAGE) is a well-established and versatile method for probing nucleic acid conformation and nucleic acid-protein interactions. Native PAGE has been used to measure RNA folding equilibria and kinetics under a wide variety of conditions. Advantages of this method are its adaptability, absolute determination of reaction endpoints, and direct analysis of conformational hetereogeneity within a sample. Native PAGE is also useful for resolving ligand-induced structural changes.

Indexed as

Electrophoresis, Polyacrylamide GelNucleic Acid ConformationRNARNA

Identifiers

PMID20946790
PMCPMC6343852
OpenAlexW41779663

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.