ArticleGenome research2010
Assessing the effect of the CLPG mutation on the microRNA catalog of skeletal muscle using high-throughput sequencing.
Article in Genome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 42 citations in OpenAlex.
- Sex-specific multigenerational epigenetic responses to real-world chemical mixture exposure in an outbred sheep model.bioRxiv : the preprint server for biology · 2026Article
- Gene expression profiles in specific skeletal muscles and meat quality characteristics of sheep and goats.Scientific data · 2024Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Role of microRNAs in myogenesis and their effects on meat quality in pig - A review.Asian-Australasian journal of animal sciences · 2020Article
- Expression analysis of lung miRNAs responding to ovine VM virus infection by RNA-seq.BMC genomics · 2019Article
- A Hearty Dose of Noncoding RNAs: The ImprintedJournal of cardiovascular development and disease · 2018Review
- Identification of genes directly responding to DLK1 signaling in Callipyge sheep.BMC genomics · 2018Article
- Article
- An Integrated Analysis of miRNAs and Methylated Genes Encoding mRNAs and lncRNAs in Sheep Breeds with Different Fecundity.Frontiers in physiology · 2017Article
- Ovarian transcriptomic study reveals the differential regulation of miRNAs and lncRNAs related to fecundity in different sheep.Scientific reports · 2016Article
- Computational identification and characterization of novel microRNA in the mammary gland of dairy goat (Capra hircus).Journal of genetics · 2016Article
- Improved transcription and translation with L-leucine stimulation of mTORC1 in Roberts syndrome.BMC genomics · 2016Article
- Identification and Expression Profiling of miRNAome in Goat longissimus dorsi Muscle from Prenatal Stages to a Neonatal Stage.PloS one · 2016Article
- Regulation of microRNA during cardiomyocyte maturation in sheep.BMC genomics · 2015Article
- Article
- Ectopic Expression of Retrotransposon-Derived PEG11/RTL1 Contributes to the Callipyge Muscular Hypertrophy.PloS one · 2015Article
- Identification and profiling of microRNAs in goat endometrium during embryo implantation.PloS one · 2015Article
- Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.BMC genomics · 2014Article
- Article
- Identification and profiling of microRNAs and their target genes from developing caprine skeletal Muscle.PloS one · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The callipyge phenotype is a monogenic muscular hypertrophy that is only expressed in heterozygous sheep receiving the CLPG mutation from their sire. The wild-type phenotype of CLPG/CLPG animals is thought to result from translational inhibition of paternally expressed DLK1 transcripts by maternally expressed miRNAs. To identify the miRNA responsible for this trans effect, we used high-throughput sequencing to exhaustively catalog miRNAs expressed in skeletal muscle of sheep of the four CLPG genotypes. We have identified 747 miRNA species of which 110 map to the DLK1-GTL2 or callipyge domain. We demonstrate that the latter are imprinted and preferentially expressed from the maternal allele. We show that the CLPG mutation affects their level of expression in cis (∼3.2-fold increase) as well as in trans (∼1.8-fold increase). In CLPG/CLPG animals, miRNAs from the DLK1-GTL2 domain account for ∼20% of miRNAs in skeletal muscle. We show that the CLPG genotype affects the levels of A-to-I editing of at least five pri-miRNAs of the DLK1-GTL2 domain, but that levels of editing of mature miRNAs are always minor. We present suggestive evidence that the miRNAs from the domain target the ORF of DLK1, thereby causing the trans inhibition underlying polar overdominance. We highlight the limitations of high-throughput sequencing for digital gene expression profiling as a result of biased and inconsistent amplification of specific miRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.