ArticleMolecular biology of the cell2010
Microautophagy of the nucleus coincides with a vacuolar diffusion barrier at nuclear-vacuolar junctions.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace.International journal of molecular sciences · 2023Review
- The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delays.Life science alliance · 2022Article
- The necessity of nucleophagic modality.Autophagy · 2022Article
- Spatial control of avidity regulates initiation and progression of selective autophagy.Nature communications · 2021Article
- Roles for LMolecular biology of the cell · 2021Article
- Article
- Mechanistic dissection of macro- and micronucleophagy.Autophagy · 2021Article
- Nutrient transceptors physically interact with the yeast S6/protein kinase B homolog, Sch9, a TOR kinase target.The Biochemical journal · 2021Article
- Autophagy of the Nucleus in Health and Disease.Frontiers in cell and developmental biology · 2021Review
- Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole.Biomolecules · 2020Article
- Nucleophagy-Implications for Microautophagy and Health.International journal of molecular sciences · 2020Review
- Article
- Review
- Lysosome biology in autophagy.Cell discovery · 2020Review
- The functional universe of membrane contact sites.Nature reviews. Molecular cell biology · 2020Review
- The Participation of Regulatory Lipids in Vacuole Homotypic Fusion.Trends in biochemical sciences · 2019Review
- Sorting the trash: Micronucleophagy gets selective.The Journal of cell biology · 2018Article
- CLIP and cohibin separate rDNA from nucleolar proteins destined for degradation by nucleophagy.The Journal of cell biology · 2018Article
- Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system.Scientific reports · 2018Article
- Mechanistic insight into the nucleus-vacuole junction based on the Vac8p-Nvj1p crystal structure.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclei bind yeast vacuoles via nucleus-vacuole (NV) junctions. Under nutrient restriction, NV junctions invaginate and release vesicles filled with nuclear material into vacuoles, resulting in piecemeal microautophagy of the nucleus (PMN). We show that the electrochemical gradient across the vacuolar membrane promotes invagination of NV junctions. Existing invaginations persist independently of the gradient, but final release of PMN vesicles requires again V-ATPase activity. We find that NV junctions form a diffusion barrier on the vacuolar membrane that excludes V-ATPase but is enriched in the VTC complex and accessible to other membrane-integral proteins. V-ATPase exclusion depends on the NV junction proteins Nvj1p,Vac8p, and the electrochemical gradient. It also depends on factors of lipid metabolism, such as the oxysterol binding protein Osh1p and the enoyl-CoA reductase Tsc13p, which are enriched in NV junctions, and on Lag1p and Fen1p. Our observations suggest that NV junctions form in two separable steps: Nvj1p and Vac8p suffice to establish contact between the two membranes. The electrochemical potential and lipid-modifying enzymes are needed to establish the vacuolar diffusion barrier, invaginate NV junctions, and form PMN vesicles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.