ArticleJournal of clinical microbiology2010
Performance of commercial enzyme-linked immunosorbent assays for detection of antibodies to Bordetella pertussis.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 94 citations in OpenAlex.
- What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Guideline
- Safety and immunogenicity of a new formulation of a pentavalent DTwP-HepB-Hib vaccine in healthy Indian infants-A randomized study.PloS one · 2023Trial
- Immunogenicity and reactogenicity of the human rotavirus vaccine, RIX4414 oral suspension, when co-administered with routine childhood vaccines in Chinese infants.Human vaccines & immunotherapeutics · 2016Trial
- Seroprevalence of diphtheria, tetanus, pertussis among healthcare workers (HCWs) - A cross-sectional study at the National Centre for Infectious Diseases and Tan Tock Seng Hospital, Singapore.Human vaccines & immunotherapeutics · 2026Article
- Pertussis resurgence: epidemiological trends, pathogenic mechanisms, and preventive strategies.Frontiers in immunology · 2025Review
- Seroepidemiology of pertussis immunity in five provinces of China: A population-based, cross-sectional study.Human vaccines & immunotherapeutics · 2024Article
- Pertussis seroprevalence in mother-infant pairs from India: role of maternal immunisation.Archives of disease in childhood · 2022Observational
- Burden of whooping cough in China (PertussisChina): study protocol of a prospective, population-based case-control study.BMJ open · 2022Article
- Hospital-based prospective study of pertussis in infants and close contacts in Tehran, Iran.BMC infectious diseases · 2021Article
- Pertussis in infants, in their mothers and other contacts in Casablanca, Morocco.BMC infectious diseases · 2020Article
- Article
- Development and validation of a robust multiplex serological assay to quantify antibodies specific to pertussis antigens.Biologicals : journal of the International Association of Biological Standardization · 2019Article
- Article
- Pertussis surveillance by small serosurveys of blood donors.Epidemiology and infection · 2018Article
- Pertussis and parapertussis in children and adults with a persistent cough: an observational study.Infection · 2018Observational
- Article
- Evaluation of Commercial Assays for Single-Point Diagnosis of Pertussis in the US.Journal of the Pediatric Infectious Diseases Society · 2017Article
- Pertussis surveillance and control: exploring variations and delays in testing, laboratory diagnostics and public health service notifications, the Netherlands, 2010 to 2013.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
- Pertussis Reinfection in an Adult: A Cause of Persistent Cough Not to Be Ignored.Case reports in infectious diseases · 2017Article
- Bordetella pertussis Infection in South African HIV-Infected and HIV-Uninfected Mother-Infant Dyads: A Longitudinal Cohort Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Measuring antibodies to Bordetella pertussis antigens is mostly done by enzyme-linked immunosorbent assays (ELISAs). We compared the performance of ELISA kits that were commercially available in Germany. Eleven measured IgG antibodies, and nine measured IgA antibodies. An in-house ELISA with purified antigens served as a reference method. Samples included two WHO reference preparations, the former Food and Drug Administration (FDA)/Center for Biologics Evaluation and Research (CBER) reference preparations, serum samples from patients with clinically suspected pertussis, and serum samples from patients having received a combined tetanus, diphtheria, and pertussis (Tdap) vaccination. Kits using pertussis toxin (PT) as an antigen showed linearity compared to the WHO Reference preparation (r2 between 0.82 and 0.99), and these kits could quantify antibodies according to the reference preparation. ELISA kits using mixed antigens showed no linear correlation to the reference preparations. Patient results were compared to results of in-house ELISAs using a dual cutoff of either ≥100 IU/ml anti-PT IgG or ≥40 IU/ml anti-PT IgG together with ≥12 IU/ml anti-PT IgA. The sensitivities of kits measuring IgG antibodies ranged between 0.84 and 1.00. The specificities of kits using PT as an antigen were between 0.81 and 0.93. The specificities of kits using mixed antigens were between 0.51 and 0.59 and were thus not acceptable. The sensitivities of kits measuring IgA antibodies ranged between 0.53 and 0.73, and the specificities were between 0.67 and 0.94, indicating that IgA antibodies may be of limited diagnostic value. Our data suggest that ELISAs should use purified PT as an antigen and be standardized to the 1st International Reference preparation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.