ArticlePlant physiology2010
Metabolic engineering of seeds can achieve levels of omega-7 fatty acids comparable with the highest levels found in natural plant sources.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Lipid regulation inFrontiers in plant science · 2026Review
- The coexpression of two desaturases provides an optimized reduction of saturates in camelina oil.Plant biotechnology journal · 2023Article
- Regioselectivity mechanism of the Thunbergia alata Δ6-16:0-acyl carrier protein desaturase.Plant physiology · 2022Article
- Signatures of selection in recently domesticated macadamia.Nature communications · 2022Article
- Molecular Approaches Reduce Saturates and EliminateFrontiers in plant science · 2022Review
- Comparison of Lipid and Palmitoleic Acid Induction ofInternational journal of molecular sciences · 2019Article
- RNA-seq data reveals a coordinated regulation mechanism of multigenes involved in the high accumulation of palmitoleic acid and oil in sea buckthorn berry pulp.BMC plant biology · 2019Article
- Stearoyl-ACP ΔFrontiers in plant science · 2019Article
- Seeds as oil factories.Plant reproduction · 2018Review
- Biotin Attachment Domain-Containing Proteins Irreversibly Inhibit Acetyl CoA Carboxylase.Plant physiology · 2018Article
- Overexpression of MYB115, AAD2, or AAD3 in Arabidopsis thaliana seeds yields contrasting omega-7 contents.PloS one · 2018Article
- Common and Species-Specific Effects of Phosphate on Marine Microalgae Fatty Acids Shape Their Function in Phytoplankton Trophic Ecology.Microbial ecology · 2017Article
- Spatial and Temporal Mapping of Key Lipid Species inPlant physiology · 2017Article
- Genetic enhancement of palmitic acid accumulation in cotton seed oil through RNAi down-regulation of ghKAS2 encoding β-ketoacyl-ACP synthase II (KASII).Plant biotechnology journal · 2017Article
- ACYL-ACYL CARRIER PROTEIN DESATURASE2 and 3 Are Responsible for Making Omega-7 Fatty Acids in the Arabidopsis Aleurone.Plant physiology · 2016Article
- Synthetic redesign of plant lipid metabolism.The Plant journal : for cell and molecular biology · 2016Review
- Vegetable Oil: Nutritional and Industrial Perspective.Current genomics · 2016Article
- Half-of-the-Sites Reactivity of the Castor Δ9-18:0-Acyl Carrier Protein Desaturase.Plant physiology · 2015Article
Corrections and comments
- Erratum issuedPidkowich, Mark S [added]
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant oils containing ω-7 fatty acids (FAs; palmitoleic 16:1Δ(9) and cis-vaccenic 18:1Δ(11)) have potential as sustainable feedstocks for producing industrially important octene via metathesis chemistry. Engineering plants to produce seeds that accumulate high levels of any unusual FA has been an elusive goal. We achieved high levels of ω-7 FA accumulation by systematic metabolic engineering of Arabidopsis (Arabidopsis thaliana). A plastidial 16:0-ACP desaturase has been engineered to convert 16:0 to 16:1Δ(9) with specificity >100-fold than that of naturally occurring paralogs, such as that from cat's claw vine (Doxantha unguis-cati). Expressing this engineered enzyme (Com25) in seeds increased ω-7 FA accumulation from <2% to 14%. Reducing competition for 16:0-ACP by down-regulating the β-ketoacyl-ACP synthase II 16:0 elongase further increased accumulation of ω-7 FA to 56%. The level of 16:0 exiting the plastid without desaturation also increased to 21%. Coexpression of a pair of fungal 16:0 desaturases in the cytosol reduced the 16:0 level to 11% and increased ω-7 FA to as much as 71%, equivalent to levels found in Doxantha seeds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.