ArticleThe Journal of biological chemistry2010
Structural and biochemical studies on the chromo-barrel domain of male specific lethal 3 (MSL3) reveal a binding preference for mono- or dimethyllysine 20 on histone H4.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- Set2 and H3K36 regulate the Drosophila male X chromosome in a context-specific manner, independent from MSL complex spreading.Genetics · 2024Article
- Set2 and H3K36 regulate thebioRxiv : the preprint server for biology · 2024Article
- The Tudor-knot Domain of KAT5 Regulates Nucleosomal Substrate Acetylation.Journal of molecular biology · 2024Article
- Hotspot Cancer Mutation Impairs KAT8-mediated Nucleosomal Histone Acetylation.Journal of molecular biology · 2024Article
- Histone Readers and Their Roles in Cancer.Cancer treatment and research · 2023Article
- The role of H3K36 methylation and associated methyltransferases in chromosome-specific gene regulation.Science advances · 2021Article
- Whole-genome sequencing reveals sex determination and liver high-fat storage mechanisms of yellowstripe goby (Mugilogobius chulae).Communications biology · 2021Article
- Evolution of the Drosophila melanogaster Chromatin Landscape and Its Associated Proteins.Genome biology and evolution · 2019Article
- The many lives of KATs - detectors, integrators and modulators of the cellular environment.Nature reviews. Genetics · 2019Review
- Reading More than Histones: The Prevalence of Nucleic Acid Binding among Reader Domains.Molecules (Basel, Switzerland) · 2018Review
- The N-terminal methyltransferase homologs NRMT1 and NRMT2 exhibit novel regulation of activity through heterotrimer formation.Protein science : a publication of the Protein Society · 2018Article
- Select human cancer mutants of NRMT1 alter its catalytic activity and decrease N-terminal trimethylation.Protein science : a publication of the Protein Society · 2017Article
- Dosage Compensation in Drosophila-a Model for the Coordinate Regulation of Transcription.Genetics · 2016Review
- MRG15 is required for pre-mRNA splicing and spermatogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Histones: at the crossroads of peptide and protein chemistry.Chemical reviews · 2015Review
- Condensin II Regulates Interphase Chromatin Organization Through the Mrg-Binding Motif of Cap-H2.G3 (Bethesda, Md.) · 2015Article
- Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.Open biology · 2014Review
- A dual role for the histone methyltransferase PR-SET7/SETD8 and histone H4 lysine 20 monomethylation in the local regulation of RNA polymerase II pausing.The Journal of biological chemistry · 2014Article
- Article
- Chromatin proteins captured by ChIP-mass spectrometry are linked to dosage compensation in Drosophila.Nature structural & molecular biology · 2013Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We have determined the human male specific lethal 3 (hMSL3) chromo-barrel domain structure by x-ray crystallography to a resolution of 2.5 Å (r = 0.226, R(free) = 0.270). hMSL3 contains a canonical methyllysine binding pocket made up of residues Tyr-31, Phe-56, Trp-59, and Trp-63. A six-residue insertion between strands β(1) and β(2) of the hMSL3 chromo-barrel domain directs the side chain of Glu-21 into the methyllysine binding pocket where it hydrogen bonds to the NH group of a bound cyclohexylamino ethanesulfonate buffer molecule, likely mimicking interactions with a histone tail dimethyllysine residue. In vitro binding studies revealed that both the human and Drosophila MSL3 chromo-barrel domains bind preferentially to peptides representing the mono or dimethyl isoform of lysine 20 on the histone H4 N-terminal tail (H4K20Me(1) or H4K20Me(2)). Mutation of Tyr-31 to Ala in the hMSL3 methyllysine-binding cage resulted in weaker in vitro binding to H4K20Me(1). The same mutation in the msl3 gene compromised male survival in Drosophila. Combined mutation of Glu-21 and Pro-22 to Ala in hMSL3 resulted in slightly weaker in vitro binding to H4K20Me(1), but the corresponding msl3 mutation had no effect on male survival in Drosophila. We propose MSL3 plays an important role in targeting the male specific lethal complex to chromatin in both humans and flies by binding to H4K20Me(1). Binding studies on the related dMRG15 chromo-barrel domain revealed that MRG15 prefers binding to H4K20Me(3).
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