ArticleThe Journal of biological chemistry2010
Structural bases of PAS domain-regulated kinase (PASK) activation in the absence of activation loop phosphorylation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Discriminating activating, deactivating and resistance variants in protein kinases.Genome medicine · 2025Article
- Structural assembly of the PAS domain drives the catalytic activation of metazoan PASK.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- 'Druggability' of the Per-Arnt-Sim (PAS) domains of human PAS domain kinase, a therapeutic target for metabolic and liver disorders.QRB discovery · 2025Article
- Nutrient Signaling-Dependent Quaternary Structure Remodeling Drives the Catalytic Activation of metazoan PASK.bioRxiv : the preprint server for biology · 2024Article
- Signal-regulated Unmasking of Nuclear Localization Motif in the PAS Domain Regulates the Nuclear Translocation of PASK.Journal of molecular biology · 2024Article
- Metabolic "Sense Relay" in Stem Cells: A Short But Impactful Life of PAS Kinase Balancing Stem Cell Fates.Cells · 2023Review
- Preventing Oxidative Stress in the Liver: An Opportunity for GLP-1 and/or PASK.Antioxidants (Basel, Switzerland) · 2021Review
- Article
- Activation of PASK by mTORC1 is required for the onset of the terminal differentiation program.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Pas Kinase Deficiency Triggers Antioxidant Mechanisms in the Liver.Scientific reports · 2018Article
- Two distinct sites of client protein interaction with the chaperone cpSRP43.The Journal of biological chemistry · 2018Article
- Pask integrates hormonal signaling with histone modification via Wdr5 phosphorylation to drive myogenesis.eLife · 2016Article
- Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production.Diabetologia · 2016Article
- Per-Arnt-Sim Kinase (PASK): An Emerging Regulator of Mammalian Glucose and Lipid Metabolism.Nutrients · 2015Review
- Molecular features of product release for the PKA catalytic cycle.Biochemistry · 2015Article
- PAS kinase drives lipogenesis through SREBP-1 maturation.Cell reports · 2014Article
- Functional divergence and evolutionary turnover in mammalian phosphoproteomes.PLoS genetics · 2014Article
- PAS kinase: a nutrient sensing regulator of glucose homeostasis.IUBMB life · 2013Review
- Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3β in pancreatic β-cells.The Journal of biological chemistry · 2013Article
- PAS kinase: integrating nutrient sensing with nutrient partitioning.Seminars in cell & developmental biology · 2012Review
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
Abstract
Per-Arnt-Sim (PAS) domain-containing protein kinase (PASK) is an evolutionary conserved protein kinase that coordinates cellular metabolism with metabolic demand in yeast and mammals. The molecular mechanisms underlying PASK regulation, however, remain unknown. Herein, we describe a crystal structure of the kinase domain of human PASK, which provides insights into the regulatory mechanisms governing catalysis. We show that the kinase domain adopts an active conformation and has catalytic activity in vivo and in vitro in the absence of activation loop phosphorylation. Using site-directed mutagenesis and structural comparison with active and inactive kinases, we identified several key structural features in PASK that enable activation loop phosphorylation-independent activity. Finally, we used combinatorial peptide library screening to determine that PASK prefers basic residues at the P-3 and P-5 positions in substrate peptides. Our results describe the key features of the PASK structure and how those features are important for PASK activity and substrate selection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.