ArticleProteome science2010
The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase.
Article in Proteome science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Impact of c-di-GMP on the Extracellular Proteome ofBiology · 2022Article
- Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15).BMC microbiology · 2022Article
- Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofThe veterinary quarterly · 2020Review
- Proteomic Analysis of Antigen 60 Complex ofVaccines · 2019Article
- Enterococcus faecalis Hydrolyzes Dental Resin Composites and Adhesives.Journal of endodontics · 2018Article
- Gallibacterium elongation factor-Tu possesses amyloid-like protein characteristics, participates in cell adhesion, and is present in biofilms.Journal of microbiology (Seoul, Korea) · 2017Article
- Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.PloS one · 2014Article
- SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins.PloS one · 2014Article
- Proteomic insights into intra- and intercellular plant-bacteria symbiotic association during root nodule formation.Frontiers in plant science · 2013Article
- Growth of Acidithiobacillus Ferrooxidans ATCC 23270 in Thiosulfate Under Oxygen-Limiting Conditions Generates Extracellular Sulfur Globules by Means of a Secreted Tetrathionate Hydrolase.Frontiers in microbiology · 2011Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe extracellular proteome or secretome of symbiotic bacteria like Rhizobium etli is presumed to be a key element of their infection strategy and survival. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. To find out the possible role of secreted proteins we analyzed the extracellular proteome of R. etli CE3 in the exponential and stationary growth phases in minimal medium, supplemented with succinate-ammonium.
resultsThe extracellular proteins were obtained by phenol extraction and identified by LC-ESI MS/MS. We identified 192 and 191 proteins for the exponential and stationary phases respectively. Using the software Signal P, we predicted signal peptides for 12.95% and 35.60% of the proteins identified in the exponential and stationary phases, respectively, which could therefore be secreted by the Sec pathway. For the exponential growth phase, we found in abundance proteins like the ribosomal proteins, toxins and proteins belonging to the group "defence mechanisms". For the stationary growth phase, we found that the most abundant proteins were those with unknown function, and in many of these we identified characteristic domains of proteases and peptidases.
conclusionsOur study provided the first dataset of the secretome of R. etli and its modifications, which may lead to novel insights into the adaptive response of different stages of growth. In addition, we found a high number of proteins with unknown function; these proteins could be analyzed in future research to elucidate their role in the extracellular proteome of R. etli.
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