ArticleBMC biotechnology2010
Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay.
Article in BMC biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
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- A Methodical Review on the Applications and Potentialities of Using Nanobiosensors for Disease Diagnosis.BioMed research international · 2022Review
- Nucleic Acids Analytical Methods for Viral Infection Diagnosis: State-of-the-Art and Future Perspectives.Biomolecules · 2021Review
- Recent advances in the detection of respiratory virus infection in humans.Journal of medical virology · 2020Review
- Diagnostic tools for tackling febrile illness and enhancing patient management.Microelectronic engineering · 2018Article
- Chiral MoSGlobal challenges (Hoboken, NJ) · 2018Article
- Applications of gold nanoparticles in virus detection.Theranostics · 2018Review
- Advanced nanotechnologies in avian influenza: Current status and future trends - A review.Analytica chimica acta · 2017Review
- Advanced Nanobiomaterials: Vaccines, Diagnosis and Treatment of Infectious Diseases.Molecules (Basel, Switzerland) · 2016Review
- Review
- Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay.Biosensors & bioelectronics · 2016Article
- Nanomicroarray and multiplex next-generation sequencing for simultaneous identification and characterization of influenza viruses.Emerging infectious diseases · 2015Article
- Nucleic-acid testing, new platforms and nanotechnology for point-of-decision diagnosis of animal pathogens.Methods in molecular biology (Clifton, N.J.) · 2015Article
- A review on emerging diagnostic assay for viral detection: the case of avian influenza virus.Molecular biology reports · 2015Review
- A highly sensitive europium nanoparticle-based immunoassay for detection of influenza A/B virus antigen in clinical specimens.Journal of clinical microbiology · 2014Article
- Rapid detection and differentiation of swine-origin influenza A virus (H1N1/2009) from other seasonal influenza A viruses.Viruses · 2012Article
- Phage displayed peptides to avian H5N1 virus distinguished the virus from other viruses.PloS one · 2011Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFor more than a decade there has been increasing interest in the use of nanotechnology and microarray platforms for diagnostic applications. In this report, we describe a rapid and simple gold nanoparticle (NP)-based genomic microarray assay for specific identification of avian influenza virus H5N1 and its discrimination from other major influenza A virus strains (H1N1, H3N2).
resultsCapture and intermediate oligonucleotides were designed based on the consensus sequences of the matrix (M) gene of H1N1, H3N2 and H5N1 viruses, and sequences specific for the hemaglutinin (HA) and neuraminidase (NA) genes of the H5N1 virus. Viral RNA was detected within 2.5 hours using capture-target-intermediate oligonucleotide hybridization and gold NP-mediated silver staining in the absence of RNA fragmentation, target amplification, and enzymatic reactions. The lower limit of detection (LOD) of the assay was less than 100 fM for purified PCR fragments and 103 TCID50 units for H5N1 viral RNA.
conclusionsThe NP-based microarray assay was able to detect and distinguish H5N1 sequences from those of major influenza A viruses (H1N1, H3N2). The new method described here may be useful for simultaneous detection and subtyping of major influenza A viruses.
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