Evidence map›Paper›PMID 20942396›Full record

ArticleBiomacromolecules2010

Layer-by-layer assembly of DNA- and protein-containing films on microneedles for drug delivery to the skin.

Eric M Saurer, Ryan M Flessner, Sean P Sullivan, Mark R Prausnitz, David M Lynn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomacromolecules, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
5.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 152 citations in OpenAlex.

  1. Review
  2. Smart micro/nanoneedles for gene delivery.Iranian journal of basic medical sciences · 2026
    Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Article
  9. Microneedle systems for delivering nucleic acid drugs.Journal of pharmaceutical investigation · 2022
    Review
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Microneedle Coating Methods: A Review with a Perspective.The Journal of pharmacology and experimental therapeutics · 2019
    Review
  17. An update on coating/manufacturing techniques of microneedles.Drug delivery and translational research · 2018
    Review
  18. Article
  19. Article
  20. Polymeric microneedles for transdermal protein delivery.Advanced drug delivery reviews · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Eric M SaurerDepartment of Chemical and Biological Engineering, 1415 Engineering Drive, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States, Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, Georgia 30332, United States, and School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, Georgia 30332, United States.
Ryan M Flessner
Sean P Sullivan
Mark R Prausnitz
David M Lynn
Georgia Institute of Technology · USUniversity of Wisconsin–Madison · US

Funding

Self-administered microneedle patches for influenza vaccinesU01AI074579 · NIAID · EMORY UNIVERSITY · PI COMPANS, RICHARD W · 2007 to 2011
$7.7M
Microneedle-based immunization against pandemic influenzaR01EB006369 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI PRAUSNITZ, MARK R. · 2007 to 2011
$3.6M
Controlled Release of DNA from SurfacesR01EB006820 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI LYNN, DAVID M · 2008 to 2011
$1.3M
NIAID NIH HHS 1 U01 AI074579NIAID NIH HHS U01 AI074579NIBIB NIH HHS 1 R01 EB006369NIBIB NIH HHS 1 R01 EB006820NIBIB NIH HHS R01 EB006369NIBIB NIH HHS R01 EB006820
6 · The paper itself

Abstract

Microneedle patches contain micrometer-scale needles coated with bioactive agents for minimally invasive drug delivery to the skin. In this study, we introduce layer-by-layer approaches to the fabrication of ultrathin DNA- and protein-containing polyelectrolyte films (or "polyelectrolyte multilayers", PEMs) on the surfaces of stainless steel microneedles. DNA-containing PEMs were fabricated on microneedles by the alternating deposition of plasmid DNA and a hydrolytically degradable poly(β-amino ester). Protein-containing PEMs were fabricated using sodium poly(styrene sulfonate) (SPS) and bovine pancreatic ribonuclease A (RNase A) conjugated to a synthetic protein transduction domain. Layer-by-layer assembly resulted in ultrathin, uniform, and defect-free coatings on the surfaces of the microneedles, as characterized by fluorescence microscopy. These films eroded and thereby released DNA or protein when incubated in saline or when inserted into porcine cadaver skin and deposited DNA or protein along the edges of microneedle tracks to depths of ∼500 to 600 μm. We conclude that PEM-coated microneedles offer a novel and useful approach to the transdermal delivery of DNA- and protein-based therapeutics and could also prove useful in other applications.

Indexed as

NeedlesAdministration, CutaneousAnimalsCattleDNADrug Delivery SystemsElectrolytesPolymersPolystyrenesRibonuclease, PancreaticSkinSwineDNAElectrolytespoly(beta-amino ester)PolymersPolystyrenespolystyrene sulfonic acidRibonuclease, Pancreatic

Identifiers

PMID20942396
PMCPMC3033977
OpenAlexW2119422370

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.