ArticleThe EMBO journal2010
Visualizing the transfer-messenger RNA as the ribosome resumes translation.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Endogenous trans-translation structure visualizes the decoding of the first tmRNA alanine codon.Frontiers in microbiology · 2024Article
- Structures of tmRNA and SmpB as they transit through the ribosome.Nature communications · 2021Article
- Ribosome Rescue Pathways in Bacteria.Frontiers in microbiology · 2021Review
- RNA structure prediction using positive and negative evolutionary information.PLoS computational biology · 2020Article
- Escherichia coli NusG Links the Lead Ribosome with the Transcription Elongation Complex.iScience · 2020Article
- Joachim Frank's Binding with the Ribosome.Structure (London, England : 1993) · 2019Review
- Structural basis for ArfA-RF2-mediated translation termination on mRNAs lacking stop codons.Nature · 2017Article
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- Mechanisms of ribosome rescue in bacteria.Nature reviews. Microbiology · 2015Review
- The bacterial translation stress response.FEMS microbiology reviews · 2014Review
- In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution.RNA (New York, N.Y.) · 2014Article
- Requirements for resuming translation in chimeric transfer-messenger RNAs of Escherichia coli and Mycobacterium tuberculosis.BMC molecular biology · 2014Article
- Biochemical aspects of bacterial strategies for handling the incomplete translation processes.Frontiers in microbiology · 2014Review
- Mechanism of trans-translation revealed by in vitro studies.Frontiers in microbiology · 2014Review
- Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB.Frontiers in microbiology · 2014Review
- tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.Frontiers in genetics · 2014Review
- Cryo-electron microscopy modeling by the molecular dynamics flexible fitting method.Biopolymers · 2012Article
- Article
- Fluorescence characterization of the transfer RNA-like domain of transfer messenger RNA in complex with small binding protein B.Biochemistry · 2012Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Bacterial ribosomes stalled by truncated mRNAs are rescued by transfer-messenger RNA (tmRNA), a dual-function molecule that contains a tRNA-like domain (TLD) and an internal open reading frame (ORF). Occupying the empty A site with its TLD, the tmRNA enters the ribosome with the help of elongation factor Tu and a protein factor called small protein B (SmpB), and switches the translation to its own ORF. In this study, using cryo-electron microscopy, we obtained the first structure of an in vivo-formed complex containing ribosome and the tmRNA at the point where the TLD is accommodated into the ribosomal P site. We show that tmRNA maintains a stable 'arc and fork' structure on the ribosome when its TLD moves to the ribosomal P site and translation resumes on its ORF. Based on the density map, we built an atomic model, which suggests that SmpB interacts with the five nucleotides immediately upstream of the resume codon, thereby determining the correct selection of the reading frame on the ORF of tmRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.