ArticlePlant physiology2010
mEosFP-based green-to-red photoconvertible subcellular probes for plants.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 68 citations in OpenAlex.
- Fingers for signaling? A possible role of stromules in intracellular communication.Plant physiology · 2026Article
- PMF proteins mediate mitochondrial fusion in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Polar localization and local translation of RHO-RELATED PROTEIN FROM PLANTS2 mRNAs promote root hair growth in Arabidopsis.The Plant cell · 2024Article
- Proximity driven plastid-nucleus relationships are facilitated by tandem plastid-ER dynamics.Journal of experimental botany · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024Review
- Using ER-Targeted Photoconvertible Fluorescent Proteins in Living Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Membrane contacts with the endoplasmic reticulum modulate plastid morphology and behaviour.Frontiers in plant science · 2023Article
- O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.Journal of experimental botany · 2022Article
- Fluorescent proteins of the EosFP clade: intriguing marker tools with multiple photoactivation modes for advanced microscopy.RSC chemical biology · 2021Review
- Periodic subcellular structures undergo long-range synchronized reorganization duringJournal of cell science · 2020Article
- Defining the dance: quantification and classification of endoplasmic reticulum dynamics.Journal of experimental botany · 2020Article
- Gateway-compatible vectors for functional analysis of proteins in cell type specific manner.Plant methods · 2020Article
- Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules.Frontiers in plant science · 2018Article
- Stromules: Probing Formation and Function.Plant physiology · 2018Review
- Applications of phototransformable fluorescent proteins for tracking the dynamics of cellular components.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2015Review
- Green to red photoconversion of GFP for protein tracking in vivo.Scientific reports · 2015Article
- Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal.Frontiers in plant science · 2015Review
- Mitochondrial pleomorphy in plant cells is driven by contiguous ER dynamics.Frontiers in plant science · 2015Article
- Mitochondrial Dynamics and the ER: The Plant Perspective.Frontiers in cell and developmental biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photoconvertible fluorescent proteins (FPs) are recent additions to the biologists' toolbox for understanding the living cell. Like green fluorescent protein (GFP), monomeric EosFP is bright green in color but is efficiently photoconverted into a red fluorescent form using a mild violet-blue excitation. Here, we report mEosFP-based probes that localize to the cytosol, plasma membrane invaginations, endosomes, prevacuolar vesicles, vacuoles, the endoplasmic reticulum, Golgi bodies, mitochondria, peroxisomes, and the two major cytoskeletal elements, filamentous actin and cortical microtubules. The mEosFP fusion proteins are smaller than GFP/red fluorescent protein-based probes and, as demonstrated here, provide several significant advantages for imaging of living plant cells. These include an ability to differentially color label a single cell or a group of cells in a developing organ, selectively highlight a region of a cell or a subpopulation of organelles and vesicles within a cell for tracking them, and understanding spatiotemporal aspects of interactions between similar as well as different organelles. In addition, mEosFP probes introduce a milder alternative to fluorescence recovery after photobleaching, whereby instead of photobleaching, photoconversion followed by recovery of green fluorescence can be used for estimating subcellular dynamics. Most importantly, the two fluorescent forms of mEosFP furnish bright internal controls during imaging experiments and are fully compatible with cyan fluorescent protein, GFP, yellow fluorescent protein, and red fluorescent protein fluorochromes for use in simultaneous, multicolor labeling schemes. Photoconvertible mEosFP-based subcellular probes promise to usher in a much higher degree of precision to live imaging of plant cells than has been possible so far using single-colored FPs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.