ArticleAnalytical chemistry2010
Synthesis of a functional metal-chelating polymer and steps toward quantitative mass cytometry bioassays.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 128 citations in OpenAlex.
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- Elemental mass spectrometry imaging of biomolecules using metal-conjugated probes.Nature reviews. Chemistry · 2025Review
- Mass spectrometric imaging and quantitative analysis of theInorganic chemistry frontiers · 2025Article
- Polymer-Based Mass Cytometry Reagents: Synthesis and Biomedical Applications.Molecules (Basel, Switzerland) · 2025Review
- Applicability of Spatial Technology in Cancer Research.Cancer research and treatment · 2024Review
- Mass Spectrometry-Based Tissue Imaging of the Tumor Microenvironment.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Highly sensitive and multiplexed mass spectrometric immunoassay techniques and clinical applications.Analytical and bioanalytical chemistry · 2022Review
- Polymeric dipicolylamine based mass tags for mass cytometry.Chemical science · 2022Article
- Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Establishing CD19 B-cell reference control materials for comparable and quantitative cytometric expression analysis.PloS one · 2021Article
- Glycosaminoglycans and Contrast Agents: The Role of Hyaluronic Acid as MRI Contrast Enhancer.Biomolecules · 2020Article
- Detection of E. coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy.Analytical and bioanalytical chemistry · 2020Article
- Immune monitoring using mass cytometry and related high-dimensional imaging approaches.Nature reviews. Rheumatology · 2020Review
- Beyond the message: advantages of snapshot proteomics with single-cell mass cytometry in solid tumors.The FEBS journal · 2019Review
- Lanthanide nanoparticles for high sensitivity multiparameter single cell analysis.Chemical science · 2019Article
- Current Trends of Microfluidic Single-Cell Technologies.International journal of molecular sciences · 2018Review
- Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry.Nature protocols · 2018Article
- Bioimaging of metallothioneins in ocular tissue sections by laser ablation-ICP-MS using bioconjugated gold nanoclusters as specific tags.Mikrochimica acta · 2017Article
- Atomic mass tag of bismuth-209 for increasing the immunoassay multiplexing capacity of mass cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2017Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We describe the synthesis and characterization of metal-chelating polymers with a degree of polymerization of 67 and 79, high diethylenetriaminepentaacetic acid (DTPA) functionality, M(w)/M(n) ≤ 1.17, and a maleimide as an orthogonal functional group for conjugation to antibodies. The polymeric disulfide form of the DP(n) = 79 DTPA polymer was analyzed by thermogravimetric analysis to determine moisture and sodium-ion content and by isothermal titration calorimetry (ITC) to determine the Gd(3+) binding capacity. These results showed each chain binds 68 ± 7 Gd(3+) per chain. Secondary goat antimouse IgG was covalently labeled with the maleimide form of the DTPA polymer (DP(n) = 79) carrying (159)Tb. Conventional ICPMS analysis of this conjugate showed each antibody carried an average of 161 ± 4 (159)Tb atoms. This result was combined with the ITC result to show there are an average of 2.4 ± 0.3 polymer chains attached to each antibody. Eleven monoclonal primary antibodies were labeled with different lanthanide isotopes using the same labeling methodology. Single cell analysis of whole umbilical cord blood stained with a mixture of 11 metal-tagged antibodies was performed by mass cytometry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.