ArticleProceedings of the National Academy of Sciences of the United States of America2010
Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 133 citations in OpenAlex.
- Microbial GAIN domains undergo autoproteolysis and enable release of diverse cell-surface-associated proteins.Current biology : CB · 2026Article
- Spatial constraints drive amylosome-mediated resistant starch degradation by Ruminococcus bromii in the human colon.Nature communications · 2025Article
- The final proteolytic step in transmembrane signaling of multiple RsgI anti-σ factors in Clostridium thermocellum.Bioscience reports · 2025Article
- Current models in bacterial hemicellulase-encoding gene regulation.Applied microbiology and biotechnology · 2024Review
- Unique Fn3-like biosensor in σProtein science : a publication of the Protein Society · 2024Article
- Extracytoplasmic polysaccharides control cellulosomal and non-cellulosomal systems in Herbivorax saccincola A7.Applied microbiology and biotechnology · 2024Article
- Insight into the autoproteolysis mechanism of the RsgI9 anti-σ factor from Clostridium thermocellum.Proteins · 2024Article
- Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors.Molecules (Basel, Switzerland) · 2024Review
- Sugar transport in thermophiles: Bridging lignocellulose deconstruction and bioconversion.Journal of industrial microbiology & biotechnology · 2024Review
- A genomic analysis reveals the diversity of cellulosome displaying bacteria.Frontiers in microbiology · 2024Article
- Structure of the transcription open complex of distinct σNature communications · 2023Article
- Bacterial SEAL domains undergo autoproteolysis and function in regulated intramembrane proteolysis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Essential autoproteolysis of bacterial anti-σ factor RsgI for transmembrane signal transduction.Science advances · 2023Article
- Bacterial SEAL domains undergo autoproteolysis and function in regulated intramembrane proteolysis.bioRxiv : the preprint server for biology · 2023Article
- Molecular Mechanism ofGenes · 2023Article
- Expression profiling of Clostridium thermocellum B8 during the deconstruction of sugarcane bagasse and straw.World journal of microbiology & biotechnology · 2023Article
- Article
- Intrinsically disordered protein regions are required for cell wall homeostasis inGenes & development · 2022Article
- The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.Proteins · 2022Article
- Article
12 more citing papers are in PubMed but not listed here.
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8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium thermocellum produces a highly efficient cellulolytic extracellular complex, termed the cellulosome, for hydrolyzing plant cell wall biomass. The composition of the cellulosome is affected by the presence of extracellular polysaccharides; however, the regulatory mechanism is unknown. Recently, we have identified in C. thermocellum a set of putative σ and anti-σ factors that include extracellular polysaccharide-sensing components [Kahel-Raifer et al. (2010) FEMS Microbiol Lett 308:84-93]. These factor-encoding genes are homologous to the Bacillus subtilis bicistronic operon sigI-rsgI, which encodes for an alternative σ(I) factor and its cognate anti-σ(I) regulator RsgI that is functionally regulated by an extracytoplasmic signal. In this study, the binding of C. thermocellum putative anti-σ(I) factors to their corresponding σ factors was measured, demonstrating binding specificity and dissociation constants in the range of 0.02 to 1 μM. Quantitative real-time RT-PCR measurements revealed three- to 30-fold up-expression of the alternative σ factor genes in the presence of cellulose and xylan, thus connecting their expression to direct detection of their extracellular polysaccharide substrates. Cellulosomal genes that are putatively regulated by two of these σ factors, σ(I1) or σ(I6), were identified based on the sequence similarity of their promoters. The ability of σ(I1) to direct transcription from the sigI1 promoter and from the promoter of celS (encodes the family 48 cellulase) was demonstrated in vitro by runoff transcription assays. Taken together, the results reveal a regulatory mechanism in which alternative σ factors are involved in regulating the cellulosomal genes via an external carbohydrate-sensing mechanism.
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