ArticleThe Journal of biological chemistry2010
Long range interactions regulate Igf2 gene transcription during skeletal muscle differentiation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Single-cell protein activity analysis reveals aberrant myogenesis and IGF2-PI3K pathway dependencies inScience advances · 2026Article
- Igf2 adult-specific skeletal muscle enhancer activity revealed in mice with intergenic CTCF boundary deletion.PLoS genetics · 2025Article
- Balancing LncRNA H19 and miR-675 Bioconversion as a Key Regulator of Embryonic Myogenesis Under Maternal Obesity.Journal of cachexia, sarcopenia and muscle · 2025Article
- Per1/Per2-Igf2 axis-mediated circadian regulation of myogenic differentiation.The Journal of cell biology · 2021Article
- Immature adipocyte-derived exosomes inhibit expression of muscle differentiation markers.FEBS open bio · 2021Article
- MasterPATH: network analysis of functional genomics screening data.BMC genomics · 2020Article
- Review
- Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing.Asian-Australasian journal of animal sciences · 2019Article
- The insulin-like growth factor 2 gene in mammals: Organizational complexity within a conserved locus.PloS one · 2019Article
- Deficiency in the nuclear long noncoding RNAThe EMBO journal · 2018Article
- Mapping human pluripotent stem cell differentiation pathways using high throughput single-cell RNA-sequencing.Genome biology · 2018Article
- Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.Scientific reports · 2018Article
- Paternal Insulin-like Growth Factor 2 (Igf2) Regulates Stem Cell Activity During Adulthood.EBioMedicine · 2017Article
- Distal regulation of c-myb expression during IL-6-induced differentiation in murine myeloid progenitor M1 cells.Cell death & disease · 2016Article
- Defining human insulin-like growth factor I gene regulation.American journal of physiology. Endocrinology and metabolism · 2016Article
- Characterizing a distal muscle enhancer in the mouse Igf2 locus.Physiological genomics · 2016Article
- Genome-Wide Detection of CNVs and Their Association with Meat Tenderness in Nelore Cattle.PloS one · 2016Article
- The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling.Genes & development · 2015Article
- Identifying growth hormone-regulated enhancers in the Igf1 locus.Physiological genomics · 2015Article
- Tbx15 controls skeletal muscle fibre-type determination and muscle metabolism.Nature communications · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The differentiation, maintenance, and repair of skeletal muscle is controlled by interactions between genetically determined transcriptional programs regulated by myogenic transcription factors and environmental cues activated by growth factors and hormones. Signaling through the insulin-like growth factor 1 (IGF1) receptor by locally produced IGF2 defines one such pathway that is critical for normal muscle growth and for regeneration after injury. IGF2 gene and protein expression are induced as early events in muscle differentiation, but the responsible molecular mechanisms are unknown. Here we characterize a distal DNA element within the imprinted mouse Igf2-H19 locus with properties of a muscle transcriptional enhancer. We find that this region undergoes a transition to open chromatin during differentiation, whereas adjacent chromatin remains closed, and that it interacts in differentiating muscle nuclei but not in mesenchymal precursor cells with the Igf2 gene found more than 100 kb away, suggesting that chromatin looping or sliding to bring the enhancer in proximity to Igf2 promoters is also an early event in muscle differentiation. Because this element directly stimulates the transcriptional activity of an Igf2 promoter-reporter gene in differentiating myoblasts, our results indicate that we have identified a bona fide distal transcriptional enhancer that supports Igf2 gene activation in skeletal muscle cells. Because this DNA element is conserved in the human IGF2-H19 locus, our results further suggest that its muscle enhancer function also is conserved among different mammalian species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.