ArticleThe Journal of biological chemistry2010
Mitotic inhibition of GRASP65 organelle tethering involves Polo-like kinase 1 (PLK1) phosphorylation proximate to an internal PDZ ligand.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Protein Kinase D2 Regulates GRASP65 Phosphorylation and Golgi Ribbon Unlinking During G2/M Transition.Cells · 2026Article
- Dichotomous roles of ACBD3 in NSCLC growth and metastasis.Oncogene · 2025Article
- Reevaluating Golgi fragmentation and its implications in wound repair.Cell regeneration (London, England) · 2024Review
- Structural Organization and Function of the Golgi Ribbon During Cell Division.Frontiers in cell and developmental biology · 2022Article
- Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation.iScience · 2021Article
- Organelle Inheritance Control of Mitotic Entry and Progression: Implications for Tissue Homeostasis and Disease.Frontiers in cell and developmental biology · 2019Review
- Golgi Structure and Function in Health, Stress, and Diseases.Results and problems in cell differentiation · 2019Review
- Global assessment of its network dynamics reveals that the kinase Plk1 inhibits the phosphatase PP6 to promote Aurora A activity.Science signaling · 2018Article
- Golgi structure formation, function, and post-translational modifications in mammalian cells.F1000Research · 2017Review
- A sequence upstream of canonical PDZ-binding motif within CFTR COOH-terminus enhances NHERF1 interaction.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Article
- Cryptic protein-protein interaction motifs in the cytoplasmic domain of MHCI proteins.BMC immunology · 2016Article
- Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2.Nature communications · 2016Article
- GRASP: A Multitasking Tether.Frontiers in cell and developmental biology · 2016Review
- Mechanisms and Regulation of the Mitotic Inheritance of the Golgi Complex.Frontiers in cell and developmental biology · 2015Review
- Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering.The Journal of biological chemistry · 2014Article
- Characterization of diverse internal binding specificities of PDZ domains by yeast two-hybrid screening of a special peptide library.PloS one · 2014Article
- Isoform-specific tethering links the Golgi ribbon to maintain compartmentalization.Molecular biology of the cell · 2014Article
- Structural insight into Golgi membrane stacking by GRASP65 and GRASP55 proteins.The Journal of biological chemistry · 2013Article
- Cell cycle regulation of Golgi membrane dynamics.Trends in cell biology · 2013Review
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2 authors.
Funding
Abstract
GRASP65 links cis-Golgi cisternae via a homotypic, N-terminal PDZ interaction, and its mitotic phosphorylation disrupts this activity. Neither the identity of the PDZ ligand involved in the GRASP65 self-interaction nor the mechanism by which phosphorylation inhibits its interaction is known. Phospho-mimetic mutation of known cyclin-dependent kinase 1/cyclin B sites, all of which are in the C-terminal "regulatory domain" of the molecule, failed to block organelle tethering. However, we identified a site phosphorylated by Polo-like kinase 1 (PLK1) in the GRASP65 N-terminal domain for which mutation to aspartic acid blocked tethering and alanine substitution prevented mitotic Golgi unlinking. Further, using interaction assays, we discovered an internal PDZ ligand adjacent to the PLK phosphorylation site that was required for tethering. These results reveal the mechanism of phosphoinhibition as direct inhibition by PLK1 of the PDZ ligand underlying the GRASP65 self-interaction.
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