ArticleThe Journal of biological chemistry2010
Role and regulation of sperm gelsolin prior to fertilization.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- Bacteria-Mediated Anomalous Rho GTPase Activation Alters Sperm Structure and Provokes Premature Capacitation Events: A Possible Mechanism of Infertility.International journal of molecular sciences · 2025Article
- Differential protein repertoires related to sperm function identified in extracellular vesicles (EVs) in seminal plasma of distinct fertility buffalo (Frontiers in cell and developmental biology · 2024Article
- Mechanisms That Protect Mammalian Sperm from the Spontaneous Acrosome Reaction.International journal of molecular sciences · 2023Review
- The Ubiquitin-Proteasome System Participates in Sperm Surface Subproteome Remodeling during Boar Sperm Capacitation.Biomolecules · 2023Article
- Proteomic Profiling of Fallopian Tube-Derived Extracellular Vesicles Using a Microfluidic Tissue-on-Chip System.Bioengineering (Basel, Switzerland) · 2023Article
- Fer and FerT: A New Regulatory Link between Sperm and Cancer Cells.International journal of molecular sciences · 2023Review
- Identification of protein candidates in spermatozoa of water buffalo (Frontiers in cell and developmental biology · 2023Article
- β-Defensin 19/119 mediates sperm chemotaxis and is associated with idiopathic infertility.Cell reports. Medicine · 2022Article
- The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.The Journal of biological chemistry · 2022Article
- The Mechanism and Experimental Validation of Forsythoside A in the Treatment of Male Infertility Were Analyzed Based on Network Pharmacology and Molecular Docking.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Ouabain-induced activation of phospholipase C zeta and its contributions to bovine sperm capacitation.Cell and tissue research · 2021Article
- Review
- Seasonal differences in seminal plasma proteins from two bovine breeds adapted to a subtropical climate.Tropical animal health and production · 2021Article
- Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability.Scientific reports · 2020Article
- Src family kinases-mediated negative regulation of sperm acrosome reaction in chickens (Gallus gallus domesticus).PloS one · 2020Article
- Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis.Journal of cell science · 2018Article
- Gelsolin dysfunction causes photoreceptor loss in induced pluripotent cell and animal retinitis pigmentosa models.Nature communications · 2017Article
- Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation.Biology open · 2016Article
- Comparative Proteomic Identification of Mature and Immature Sperm in the Catfish Cranoglanis bouderius.PloS one · 2016Article
- PKA-dependent phosphorylation of LIMK1 and Cofilin is essential for mouse sperm acrosomal exocytosis.Developmental biology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To acquire fertilization competence, spermatozoa should undergo several biochemical changes in the female reproductive tract, known as capacitation. The capacitated spermatozoon can interact with the egg zona pellucida resulting in the occurrence of the acrosome reaction, a process that allowed its penetration into the egg and fertilization. Sperm capacitation requires actin polymerization, whereas F-actin must disperse prior to the acrosome reaction. Here, we suggest that the actin-severing protein, gelsolin, is inactive during capacitation and is activated prior to the acrosome reaction. The release of bound gelsolin from phosphatidylinositol 4,5-bisphosphate (PIP(2)) by PBP10, a peptide containing the PIP(2)-binding domain of gelsolin, or by activation of phospholipase C, which hydrolyzes PIP(2), caused rapid Ca(2+)-dependent F-actin depolymerization as well as enhanced acrosome reaction. Using immunoprecipitation assays, we showed that the tyrosine kinase SRC and gelsolin coimmunoprecipitate, and activating SRC by adding 8-bromo-cAMP (8-Br-cAMP) enhanced the amount of gelsolin in this precipitate. Moreover, 8-Br-cAMP enhanced tyrosine phosphorylation of gelsolin and its binding to PIP(2(4,5)), both of which inactivated gelsolin, allowing actin polymerization during capacitation. This actin polymerization was blocked by inhibiting the Src family kinases, suggesting that gelsolin is activated under these conditions. These results are further supported by our finding that PBP10 was unable to cause complete F-actin breakdown in the presence of 8-Br-cAMP or vanadate. In conclusion, inactivation of gelsolin during capacitation occurs by its binding to PIP(2) and tyrosine phosphorylation by SRC. The release of gelsolin from PIP(2) together with its dephosphorylation enables gelsolin activation, resulting in the acrosome reaction.
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