ArticleMolecular and cellular biology2010
The protein kinase C cascade regulates recruitment of matrix metalloprotease 9 to podosomes and its release and activation.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Protein kinase C theta: evolution, regulation, and function.The Biochemical journal · 2026Review
- Pan-cancer multi-omics dissection of podosome-related genes reveals their dual roles in tumor invasion and immune modulation.Discover oncology · 2026Article
- Chemical and biological study of aplysiatoxin derivatives showing inhibition of potassium channel Kv1.5.RSC advances · 2019Article
- NEDD9 stimulated MMP9 secretion is required for invadopodia formation in oral squamous cell carcinoma.Oncotarget · 2018Article
- Matrix Metalloproteinases -14, -9 and -2 are Localized to the Podosome and Involved in Podosome Development in the A7r5 Smooth Muscle Cell.Journal of cardiobiology · 2017Article
- Stimulus-dependent dissociation between XB130 and Tks5 scaffold proteins promotes airway epithelial cell migration.Oncotarget · 2016Article
- Review
- Inhibition of protein kinase C by isojacareubin suppresses hepatocellular carcinoma metastasis and induces apoptosis in vitro and in vivo.Scientific reports · 2015Article
- XB130 translocation to microfilamentous structures mediates NNK-induced migration of human bronchial epithelial cells.Oncotarget · 2015Article
- The roles of akt isoforms in the regulation of podosome formation in fibroblasts and extracellular matrix invasion.Cancers · 2015Article
- Arf1 and Arf6 promote ventral actin structures formed by acute activation of protein kinase C and Src.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Protein kinase C mediates enterohemorrhagic Escherichia coli O157:H7-induced attaching and effacing lesions.Infection and immunity · 2014Article
- Palladin promotes invasion of pancreatic cancer cells by enhancing invadopodia formation in cancer-associated fibroblasts.Oncogene · 2014Article
- Protein kinase C, focal adhesions and the regulation of cell migration.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Review
- Spatiotemporal organization and mechanosensory function of podosomes.Cell adhesion & migration · 2014Review
- Downregulated Krüppel-like factor 8 is involved in decreased trophoblast invasion under hypoxia-reoxygenation conditions.Reproductive sciences (Thousand Oaks, Calif.) · 2014Article
- Palladin regulation of the actin structures needed for cancer invasion.Cell adhesion & migration · 2014Review
- p53 in cell invasion, podosomes, and invadopodia.Cell adhesion & migration · 2014Review
- S137 phosphorylation of profilin 1 is an important signaling event in breast cancer progression.PloS one · 2014Article
- Atypical protein kinase C in cell motility.Cellular and molecular life sciences : CMLS · 2013Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Podosomes are transient cell surface structures essential for degradation of extracellular matrix during cell invasion. Protein kinase C (PKC) is involved in the regulation of podosome formation; however, the roles of individual PKC isoforms in podosome formation and proteolytic function are largely unknown. Recently, we reported that PDBu, a PKC activator, induced podosome formation in normal human bronchial epithelial cells. Here, we demonstrate that phorbol-12,13-dibutyrate (PDBu)-induced podosome formation is mainly mediated through redistribution of conventional PKCs, especially PKCα, from the cytosol to the podosomes. Interestingly, although blocking atypical PKCζ did not affect PDBu-induced podosome formation, it significantly reduced matrix degradation at podosomes. Inhibition of PKCζ reduced recruitment of matrix metalloprotease 9 (MMP-9) to podosomes and its release and activation. Downregulation of MMP-9 by small interfering RNA (siRNA) or neutralization antibody also significantly reduced matrix degradation. The regulatory effects of PKCζ on matrix degradation and recruitment of MMP-9 to podosomes were PKCζ kinase activity dependent. PDBu-induced recruitment of PKCζ and MMP-9 to podosomes was blocked by inhibition of novel PKC with rottlerin or PKCδ siRNA. Our data suggest that multiple PKC isozymes form a signaling cascade that controls podosome formation and dynamics and MMP-9 recruitment, release, and activation in a coordinated fashion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.