ArticleAnalytical chemistry2010
Using triplex-forming oligonucleotide probes for the reagentless, electrochemical detection of double-stranded DNA.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Cu-based metal-organic frameworks: synthesis, biomedical applications, and beyond.Discover nano · 2026Review
- Chemometrics-Assisted Enhancement of Electrochemical Biosensor Performance toward miRNA Detection.Analytical chemistry · 2025Article
- Printed Electrochemical Strip for the Detection of miRNA-29a: A Possible Biomarker Related to Alzheimer's Disease.Analytical chemistry · 2022Article
- Three's a crowd - stabilisation, structure, and applications of DNA triplexes.Chemical science · 2022Review
- Applications of triplex DNA nanostructures in sensor development.Analytical and bioanalytical chemistry · 2022Review
- Exploration of intramolecular split G-quadruplex and its analytical applications.Nucleic acids research · 2019Article
- Biological and nanotechnological applications using interactions between ionic liquids and nucleic acids.Biophysical reviews · 2018Review
- Facile fabrication of microfluidic surface-enhanced Raman scattering devices via lift-up lithography.Royal Society open science · 2018Article
- Understanding Signal and Background in a Thermally Resolved, Single-Branched DNA Assay Using Square Wave Voltammetry.Analytical chemistry · 2018Article
- Metal-organic frameworks as biosensors for luminescence-based detection and imaging.Interface focus · 2016Review
- Triplex DNA: A new platform for polymerase chain reaction-based biosensor.Scientific reports · 2015Article
- Sensitive and direct electrochemical detection of double-stranded DNA utilizing alkaline phosphatase-labelled zinc finger proteins.The Analyst · 2015Article
- Monitoring cooperative binding using electrochemical DNA-based sensors.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Folding-upon-binding and signal-on electrochemical DNA sensor with high affinity and specificity.Analytical chemistry · 2014Article
- Comparable stability of Hoogsteen and Watson-Crick base pairs in ionic liquid choline dihydrogen phosphate.Scientific reports · 2014Article
- Microfluidic chip-based detection and intraspecies strain discrimination of Salmonella serovars derived from whole blood of septic mice.Applied and environmental microbiology · 2013Article
- Regulation of transcription through light-activation and light-deactivation of triplex-forming oligonucleotides in mammalian cells.ACS chemical biology · 2012Article
- Highly sensitive disposable nucleic acid biosensors for direct bioelectronic detection in raw biological samples.Talanta · 2011Article
- Evaluating the global CpG methylation status of native DNA utilizing a bipartite split-luciferase sensor.Analytical chemistry · 2011Article
- Fabrication of electrochemical-DNA biosensors for the reagentless detection of nucleic acids, proteins and small molecules.Journal of visualized experiments : JoVE · 2011Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We report a reagentless, electrochemical sensor for the detection of double-stranded DNA targets that employs triplex-forming oligonucleotides (TFOs) as its recognition element. These sensors are based on redox-tagged TFO probes strongly chemisorbed onto an interrogating gold electrode. Upon the addition of the relevant double-stranded DNA target, the probe forms a rigid triplex structure via reverse Hoogsteen base pairing in the major groove. The formation of the triplex impedes contact between the probe's redox moiety and the interrogating electrode, thus signaling the presence of the target. We first demonstrated the proof of principle of this approach by using a well-characterized 22-base polypurine TFO sequence that readily detects a synthetic, double-stranded DNA target. We then confirmed the generalizability of our platform with a second probe, a 19-base polypyrimidine TFO sequence that targets a polypurine tract (PPT) sequence conserved in all HIV-1 strains. Both sensors rapidly and specifically detect their double-stranded DNA targets at concentrations as low as ~10 nM and are selective enough to be employed directly in complex sample matrices such as blood serum. Moreover, to demonstrate real-world applicability of this new sensor platform, we have successfully detected unpurified, double-stranded PCR amplicons containing the relevant conserved HIV-1 sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.