ArticleDevelopmental neurobiology2011
Wnt/calcium signaling mediates axon growth and guidance in the developing corpus callosum.
Article in Developmental neurobiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 98 citations in OpenAlex.
- Regulation and function of specialized membrane protrusions in intercellular communication.Nature reviews. Molecular cell biology · 2026Review
- Cdk7 promotes neuritogenesis in cortical neurons and contributes to social behavior in mice.Cellular and molecular life sciences : CMLS · 2026Article
- Beyond synapses: cytoplasmic connections in brain function and evolution.Biological reviews of the Cambridge Philosophical Society · 2025Review
- How Growth Cones Sense Extracellular Cues and Drive Neuronal Migration: Shared Mechanisms Between Growth Cones of Migrating Neurons and Axons.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Engineering human cerebral organoids to explore mechanisms of arsenic-induced developmental neurotoxicity.Toxicology and applied pharmacology · 2025Article
- Wnt5a Promotes Axon Elongation in Coordination with the Wnt-Planar Cell Polarity Pathway.Cells · 2024Article
- Spatiotemporal changes in Netrin/Dscam1 signaling dictate axonal projection direction ineLife · 2024Article
- A loss-of-function mutation in human Oxidation Resistance 1 disrupts the spatial-temporal regulation of histone arginine methylation in neurodevelopment.Genome biology · 2023Article
- A Role for Second Messengers in Axodendritic Neuronal Polarity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Review
- Celsr1 suppresses Wnt5a-mediated chemoattraction to prevent incorrect rostral migration of facial branchiomotor neurons.Development (Cambridge, England) · 2022Article
- Involvement of Calcium-Dependent Pathway and β Subunit-Interaction in Neuronal Migration and Callosal Projection Deficits Caused by the Cav1.2 I1166T Mutation in Developing Mouse Neocortex.Frontiers in neuroscience · 2021Article
- The +TIP Navigator-1 is an actin-microtubule crosslinker that regulates axonal growth cone motility.The Journal of cell biology · 2020Article
- The effect of extrinsic Wnt/β-catenin signaling in Muller glia on retinal ganglion cell neurite growth.Developmental neurobiology · 2020Article
- The regulation of mitochondrial dynamics in neurite outgrowth by retinoic acid receptor β signaling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model.Neural plasticity · 2019Article
- A Subtle Network Mediating Axon Guidance: Intrinsic Dynamic Structure of Growth Cone, Attractive and Repulsive Molecular Cues, and the Intermediate Role of Signaling Pathways.Neural plasticity · 2019Review
- Wnt signalling in the development of axon, dendrites and synapses.Open biology · 2018Review
- RPM-1 and DLK-1 regulate pioneer axon outgrowth by controlling Wnt signaling.Development (Cambridge, England) · 2018Article
- Radial WNT5A-Guided Post-mitotic Filopodial Pathfinding Is Critical for Midgut Tube Elongation.Developmental cell · 2018Article
- The Microtubule-Associated Protein Tau Mediates the Organization of Microtubules and Their Dynamic Exploration of Actin-Rich Lamellipodia and Filopodia of Cortical Growth Cones.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It has been shown in vivo that Wnt5a gradients surround the corpus callosum and guide callosal axons after the midline (postcrossing) by Wnt5a-induced repulsion via Ryk receptors. In dissociated cortical cultures we showed that Wnt5a simultaneously promotes axon outgrowth and repulsion by calcium signaling. Here to test the role of Wnt5a/calcium signaling in a complex in vivo environment we used sensorimotor cortical slices containing the developing corpus callosum. Plasmids encoding the cytoplasmic marker DsRed and the genetically encoded calcium indicator GCaMP2 were electroporated into one cortical hemisphere. Postcrossing callosal axons grew 50% faster than pre-crossing axons and higher frequencies of calcium transients in axons and growth cones correlated well with outgrowth. Application of pharmacological inhibitors to the slices showed that signaling pathways involving calcium release through IP3 receptors and calcium entry through TRP channels regulate post-crossing axon outgrowth and guidance. Co-electroporation of Ryk siRNA and DsRed revealed that knock down of the Ryk receptor reduced outgrowth rates of postcrossing but not precrossing axons by 50% and caused axon misrouting. Guidance errors in axons with Ryk knockdown resulted from reduced calcium activity. In the corpus callosum CaMKII inhibition reduced the outgrowth rate of postcrossing (but not precrossing) axons and caused severe guidance errors which resulted from reduced CaMKII-dependent repulsion downstream of Wnt/calcium. We show for the first time that Wnt/Ryk calcium signaling mechanisms regulating axon outgrowth and repulsion in cortical cultures are also essential for the proper growth and guidance of postcrossing callosal axons which involve axon repulsion through CaMKII.
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Registered trials
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