ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2011
Universal PCR assays for the differential detection of all Old World Leishmania species.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- SERS-based spectral analysis of ITS1 PCR products for differentiation of three old world Leishmania species.Lasers in medical science · 2026Article
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- Artificial Intelligence in Cutaneous Leishmaniasis Diagnosis: Current Developments and Future Perspectives.Diagnostics (Basel, Switzerland) · 2024Review
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- Genome diversity ofFrontiers in cellular and infection microbiology · 2023Article
- Molecular Characterization ofPathogens (Basel, Switzerland) · 2022Article
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- Determinants of Unresponsiveness to Treatment in Cutaneous Leishmaniasis: A Focus on Anthroponotic Form Due toFrontiers in microbiology · 2021Review
- Tabanids as possible pathogen vectors in Senegal (West Africa).Parasites & vectors · 2020Article
- Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis.Parasites & vectors · 2020Article
- Clinical, parasitological and molecular profiles of Cutaneous Leishmaniasis and its associated factors among clinically suspected patients attending Borumeda Hospital, North-East Ethiopia.PLoS neglected tropical diseases · 2020Article
- Evaluation of a pan-Leishmania SL RNA qPCR assay for parasite detection in laboratory-reared and field-collected sand flies and reservoir hosts.Parasites & vectors · 2020Article
- New Molecular Approach for the Detection of Kinetoplastida Parasites of Medical and Veterinary Interest.Microorganisms · 2020Article
- Cutaneous Leishmaniasis Due toEClinicalMedicine · 2018Review
- Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP.Journal of arthropod-borne diseases · 2018Article
- Leishmania major and Trypanosoma lewisi infection in invasive and native rodents in Senegal.PLoS neglected tropical diseases · 2018Article
- Analytical Performance of Four Polymerase Chain Reaction (PCR) and Real Time PCR (qPCR) Assays for the Detection of SixFrontiers in microbiology · 2017Article
- Detection, molecular typing and phylogenetic analysis ofParasite epidemiology and control · 2016Article
- Localized Leishmania Lymphadenitis Etiologic Agent Identified as Leishmania tropica Using Gene Sequencing.Iranian journal of pathology · 2016Article
- Cutaneous leishmaniasis: recent developments in diagnosis and management.American journal of clinical dermatology · 2015Review
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the epidemiological monitoring and clinical case management of leishmaniasis, determination of the causative Leishmania species gains importance. Current assays for the Old World often suffer from drawbacks in terms of validation on a geographically representative sample set and the ability to recognize all species complexes. We want to contribute to standardized species typing for Old World leishmaniasis. We determined the ribosomal DNA internal transcribed spacer 1 sequence of 24 strains or isolates, and validated four species-specific polymerase chain reactions (PCRs) amplifying this target. They discriminate L. aethiopica, L. tropica, L. major, and the L. donovani complex, use the same cycling conditions, and include an internal amplification control. Our PCRs amplify 0.1 pg of Leishmania DNA, while being 100% specific for species identification on an extensive panel of geographically representative strains and isolates. Similar results were obtained in an endemic reference laboratory in Kenya. Species could also be identified in clinical specimens. The presented PCRs require only agarose gel detection, and have several other advantages over many existing assays. We outline potential problems, suggest concrete solutions for transferring the technique to other settings, and deliver the proof-of-principle for analyzing clinical samples.
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