ArticleInternational journal of microbiology2010
Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.
Article in International journal of microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 43 citations in OpenAlex.
- Serotonin modulates insect gut bacterial community homeostasis.BMC biology · 2022Article
- Molecular Tools To Study Preharvest Food Safety Challenges.Microbiology spectrum · 2018Review
- Development of a Droplet Digital Polymerase Chain Reaction for Rapid and Simultaneous Identification of Common Foodborne Pathogens in Soft Cheese.Frontiers in microbiology · 2016Article
- Customizable PCR-microplate array for differential identification of multiple pathogens.Journal of food protection · 2013Article
- Novel genomic tools for specific and real-time detection of biothreat and frequently encountered foodborne pathogens.Journal of food protection · 2012Article
- Development of a microbial high-throughput screening instrument based on elastic light scatter patterns.The Review of scientific instruments · 2012Article
- Yersinia enterocolitica and Yersinia pseudotuberculosis Detection in Foods.Journal of pathogens · 2011Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of 8 multiplex real-time SYBR Green PCR (SG-PCR) assays including 3 target primers and an internal amplification control (IAC) primer was simultaneously evaluated in 3 h or less with regard to detection of 24 target genes of 23 foodborne pathogens in 7 stool specimens of foodborne outbreak using a 96-well reaction plate. This assay, combined with DNA extraction (QIAamp DNA Stool Mini kit), offered detection of greater than 10(3)-10(4) foodborne pathogens per g in stool specimens. The products formed were identified using melting point temperature (Tm) curve analysis. This assay was evaluated for the detection of foodborne pathogens in 33 out of 35 cases of foodborne outbreak, using 4 different PCR instruments in 5 different laboratories. No interference from the multiplex real-time SG-PCR assay, including IAC, was observed in stool specimens in any analysis. We found multiplex real-time SG-PCR assay for simultaneous detection of 24 target genes of foodborne pathogens to be comprehensive, rapid, inexpensive, accurate, of high selectivity, and good for screening probability.
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Registered trials
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