ArticleCell cycle (Georgetown, Tex.)2010
Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4.
Article in Cell cycle (Georgetown, Tex.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 40 citations in OpenAlex.
- The multifaceted functions of SPC25 in cancer: from molecular pathways to targeted therapy.Frontiers in medicine · 2025Review
- Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.Scientific reports · 2024Article
- Twenty years of merotelic kinetochore attachments: a historical perspective.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
- Tandem affinity purification protocol for isolation of protein complexes fromSTAR protocols · 2022Article
- Mechanisms of chromosome biorientation and bipolar spindle assembly analyzed by computational modeling.eLife · 2020Article
- The molecular basis of monopolin recruitment to the kinetochore.Chromosoma · 2019Article
- The budding-yeast RWD protein Csm1 scaffolds diverse protein complexes through a conserved structural mechanism.Protein science : a publication of the Protein Society · 2018Article
- Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.Molecular biology of the cell · 2018Article
- The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.The Journal of cell biology · 2017Article
- MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast.Molecular biology of the cell · 2017Article
- Structure of the Saccharomyces cerevisiae Hrr25:Mam1 monopolin subcomplex reveals a novel kinase regulator.The EMBO journal · 2016Article
- Cutting edge science: Laser surgery illuminates viscoelasticity of merotelic kinetochores.The Journal of cell biology · 2016Article
- Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore.The Journal of cell biology · 2016Article
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- Aurora B prevents chromosome arm separation defects by promoting telomere dispersion and disjunction.The Journal of cell biology · 2015Article
- Article
- The cockayne syndrome B protein is essential for neuronal differentiation and neuritogenesis.Cell death & disease · 2014Article
- Real-time imaging of DNA damage in yeast cells using ultra-short near-infrared pulsed laser irradiation.PloS one · 2014Article
- Monopolin recruits condensin to organize centromere DNA and repetitive DNA sequences.Molecular biology of the cell · 2013Article
- Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.Molecular & cellular proteomics : MCP · 2013Article
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7 authors at 3 institutions in 3 countries.
Funding
Abstract
In order to segregate chromosomes properly, the cell must prevent merotelic kinetochore attachment, an error that occurs when a single kinetochore is attached to microtubules emanating from both spindle poles. Merotelic kinetochore orientation represents a major mechanism of aneuploidy in mitotic mammalian cells and it is the primary mechanism of chromosome instability in cancer cells. Fission yeast mutants defective in putative microtubule-site clamp Pcs1/Mde4 or Clr4/Swi6-dependent centromeric heterochromatin display high frequencies of lagging chromosomes during anaphase. Here, we developed an assay based on laser microsurgery to show that the stretched morphology of lagging kinetochores in pcs1Δ and clr4Δ mutant cells is due to merotelic attachment. We further show that Mde4 is regulated by Cdc2 and that Cdc2 activity prevents precocious localization of Mde4 to the metaphase spindle. Finally, we show that Pcs1/Mde4 complex shares similar features with the conserved kinetochore complex Spc24/Spc25 suggesting that these two complexes may occupy a similar functional niche.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.