ArticleAmerican journal of respiratory cell and molecular biology2011
Epithelial cells from smokers modify dendritic cell responses in the context of influenza infection.
Article in American journal of respiratory cell and molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Pooled it
- High-throughput bioprinting of the nasal epithelium using patient-derived nasal epithelial cells.Biofabrication · 2023Article
- In Vivo and In Vitro Studies of Cigarette Smoke Effects on Innate Responses to Influenza Virus: A Matter of Models?Viruses · 2022Review
- Small Molecule Antipsychotic Aripiprazole Potentiates Ozone-Induced Inflammation in Airway Epithelium.Chemical research in toxicology · 2019Article
- Alternative Experimental Models for Studying Influenza Proteins, Host-Virus Interactions and Anti-Influenza Drugs.Pharmaceuticals (Basel, Switzerland) · 2019Review
- Flavored e-cigarette liquids and cinnamaldehyde impair respiratory innate immune cell function.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- HPV16 E7 expression in skin induces TSLP secretion, type 2 ILC infiltration and atopic dermatitis-like lesions.Immunology and cell biology · 2015Article
- Cigarette smoke attenuates the RIG-I-initiated innate antiviral response to influenza infection in two murine models.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Article
- Culturing of human nasal epithelial cells at the air liquid interface.Journal of visualized experiments : JoVE · 2013Article
- DNA methylation in nasal epithelial cells from smokers: identification of ULBP3-related effects.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Ozone exposed epithelial cells modify cocultured natural killer cells.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Article
- Influenza enhances caspase-1 in bronchial epithelial cells from asthmatic volunteers and is associated with pathogenesis.The Journal of allergy and clinical immunology · 2012Article
- Live attenuated influenza virus (LAIV) induces different mucosal T cell function in nonsmokers and smokers.Clinical immunology (Orlando, Fla.) · 2012Article
- Alteration of the nasal responses to influenza virus by tobacco smoke.Current opinion in allergy and clinical immunology · 2012Review
- Epithelial cells, the "switchboard" of respiratory immune defense responses: effects of air pollutants.Swiss medical weekly · 2012Review
- Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus.Respiratory research · 2011Article
- Innate immunity in the respiratory epithelium.American journal of respiratory cell and molecular biology · 2011Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Epidemiologic evidence suggests that cigarette smoking is a risk factor for infection with influenza, but the mechanisms underlying this susceptibility remain unknown. To ascertain if airway epithelial cells from smokers demonstrate a decreased ability to orchestrate an influenza-induced immune response, we established a model using differentiated nasal epithelial cells (NECs) from nonsmokers and smokers, co-cultured with peripheral blood monocyte-derived dendritic cells (mono-DCs) from nonsmokers. NEC/mono-DC co-cultures were infected with influenza A virus and analyzed for influenza-induced immune responses 24 hours after infection. We observed that NECs from smokers, as well as mono-DCs co-cultured with NECs from smokers, exhibited suppressed influenza-induced, interferon-related proteins interferon regulatory factor-7, Toll-like receptor-3, and retinoic acid inducible gene-1, likely because of the suppressed production of IFNα from the NECs of smokers. Furthermore, NEC/mono-DC co-cultures using NECs from smokers exhibited suppressed concentrations of T-cell/natural killer cell chemokine interferon gamma-induced protein 10 (IP-10) after infection with influenza, indicating that NECs from smokers may skew early influenza-induced Th1 responses. In contrast, NEC/mono-DC co-cultures using NEC from smokers contained increased influenza-induced concentrations of the Th2 chemokine thymic stromal lymphopoeitin (TSLP). In addition, NECs from smokers cultured alone had increased influenza-induced concentrations of the Th2 chemokine thymus and activation-regulated chemokine (TARC). Using this model, we demonstrated that in the context of infection with influenza, NECs obtained from smokers create an overall cytokine microenvironment that suppresses the interferon-mediated Th1 response and enhances the TSLP-TARC-mediated Th2 response, with the potential to modify the responses of DCs. Smoking-induced alterations in the Th1/Th2 balance may play a role in developing underlying susceptibilities to respiratory viral infections, and may also promote the likelihood of acquiring Th2 proallergic diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.