ArticlePlant physiology2010
Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 201 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
201 citing papers in PubMed, 509 citations in OpenAlex.
- Light-dependent processes in algae across scales: from cellular physiology to ecological organization.Plant physiology · 2026Review
- Light and ABA signaling are involved in transcriptional regulation of PsbS in plants.Plant cell reports · 2026Article
- Identification and Overexpression of Endogenous Transcription Factors to Enhance Lipid Accumulation in the Commercially Relevant SpeciesbioRxiv : the preprint server for biology · 2025Article
- Biological and Nutritional Applications of Microalgae.Nutrients · 2024Review
- α/β hydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation.National science review · 2024Article
- Latitudinal patterns in ocean C:N:P reflect phytoplankton acclimation and macromolecular composition.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Towards maximizing biomass and lipid productivity: high-throughput screening assay for prospecting heterotrophic growth for new microalgal isolates.Microbial cell factories · 2024Article
- Aggregation-Induced Emission Photosensitizer Boosting Algal Growth and Lipid Accumulation.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Heterotrophic bacteria trigger transcriptome remodelling in the photosynthetic picoeukaryote Micromonas commoda.Environmental microbiology reports · 2024Article
- Nanopore Direct RNA Sequencing Reveals the Short-Term Salt Stress Response in Maize Roots.Plants (Basel, Switzerland) · 2024Article
- ROS-mediated thylakoid membrane remodeling and triacylglycerol biosynthesis under nitrogen starvation in the algaFrontiers in plant science · 2024Article
- Strategies of NaCl Tolerance in Saline-Alkali-Tolerant Green MicroalgaPlants (Basel, Switzerland) · 2023Article
- Biodiesel Production from the Marine AlgaPlants (Basel, Switzerland) · 2023Article
- Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell.Communications biology · 2023Article
- Physiological and Genetic Regulation for High Lipid Accumulation byMicrobiology spectrum · 2022Article
- Development of a stable semi-continuous lipid production system of an oleaginous Chlamydomonas sp. mutant using multi-omics profiling.Biotechnology for biofuels and bioproducts · 2022Article
- Genetic compensation of triacylglycerol biosynthesis in the green microalga Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2022Article
- 13C-labeling reveals how membrane lipid components contribute to triacylglycerol accumulation in Chlamydomonas.Plant physiology · 2022Article
- Transcriptomic and fatty acid analyses of Neochloris aquatica grown under different nitrogen concentration.Functional & integrative genomics · 2022Article
- Enhanced accumulation of oil through co-expression of fatty acid and ABC transporters in Chlamydomonas under standard growth conditions.Biotechnology for biofuels and bioproducts · 2022Article
141 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Like many microalgae, Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient deprived. To begin studying the mechanisms underlying this process, nitrogen (N) deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global analysis of transcripts under induced and noninduced conditions was applied as a first approach to studying molecular changes that promote or accompany triacylglycerol accumulation in cells encountering a new nutrient environment. Towards this goal, high-throughput sequencing technology was employed to generate large numbers of expressed sequence tags of eight biologically independent libraries, four for each condition, N replete and N deprived, allowing a statistically sound comparison of expression levels under the two tested conditions. As expected, N deprivation activated a subset of control genes involved in gametogenesis while down-regulating protein biosynthesis. Genes for components of photosynthesis were also down-regulated, with the exception of the PSBS gene. N deprivation led to a marked redirection of metabolism: the primary carbon source, acetate, was no longer converted to cell building blocks by the glyoxylate cycle and gluconeogenesis but funneled directly into fatty acid biosynthesis. Additional fatty acids may be produced by membrane remodeling, a process that is suggested by the changes observed in transcript abundance of putative lipase genes. Inferences on metabolism based on transcriptional analysis are indirect, but biochemical experiments supported some of these deductions. The data provided here represent a rich source for the exploration of the mechanism of oil accumulation in microalgae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.