ArticleApplied and environmental microbiology2010
Development of a versatile procedure based on natural transformation for marker-free targeted genetic modification in Streptococcus thermophilus.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Type III CRISPR-Cas systems preferentially acquire spacers from early-expressed phage genes in a transcription-dependent manner.Nucleic acids research · 2026Article
- Selective degradation of phage RNAs by the Csm6 ribonuclease provides robust type III CRISPR immunity in Streptococcus thermophilus.Nucleic acids research · 2024Article
- Advances in Genetic Tools and Their Application inFoods (Basel, Switzerland) · 2023Review
- Bacteriophage-host interactions in Streptococcus thermophilus and their impact on co-evolutionary processes.FEMS microbiology reviews · 2023Review
- Investigation of CRISPR-Independent Phage Resistance Mechanisms Reveals a Role for FtsH in Phage Adsorption to Streptococcus thermophilus.Journal of bacteriology · 2023Article
- Development of a Tetracycline-Inducible System for Conditional Gene Expression in Lactococcus lactis and Streptococcus thermophilus.Microbiology spectrum · 2023Article
- Article
- Expanding natural transformation to improve beneficial lactic acid bacteria.FEMS microbiology reviews · 2022Article
- Competence shut-off by intracellular pheromone degradation in salivarius streptococci.PLoS genetics · 2022Article
- Article
- Unique properties of spacer acquisition by the type III-A CRISPR-Cas system.Nucleic acids research · 2022Article
- Host-encoded, cell surface-associated exopolysaccharide required for adsorption and infection by lactococcal P335 phage subtypes.Frontiers in microbiology · 2022Article
- Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A mutation in the methionine aminopeptidase gene provides phage resistance in Streptococcus thermophilus.Scientific reports · 2019Article
- Article
- Intensive targeting of regulatory competence genes by transposable elements in streptococci.Molecular genetics and genomics : MGG · 2019Article
- Induction of Natural Competence in Genetically-modifiedBio-protocol · 2018Article
- Unleashing Natural Competence in Lactococcus lactis by Induction of the Competence Regulator ComX.Applied and environmental microbiology · 2017Article
- Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2.Applied and environmental microbiology · 2017Article
- Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS.PLoS pathogens · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A versatile natural transformation protocol was established for and successfully applied to 18 of the 19 Streptococcus thermophilus strains tested. The efficiency of the protocol enables the use of in vitro-amplified mutagenesis fragments to perform deletion or insertion of large genetic fragments. Depending on the phenotype linked to the mutation, markerless mutants can be selected either in two steps, i.e., resistance marker insertion and excision using an adapted Cre-loxP system, or in one step using a powerful positive screening procedure as illustrated here for histidine prototrophy.
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