ArticleApplied and environmental microbiology2010
Detection of saxitoxin-producing cyanobacteria and Anabaena circinalis in environmental water blooms by quantitative PCR.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 128 citations in OpenAlex.
- Article
- Diverse Cyanopeptides follow distinct temporal succession patterns in freshwater harmful algal blooms.The ISME journal · 2026Article
- Article
- Genomic Identification and Characterization of Saxitoxin Producing Cyanobacteria in Western Lake Erie Harmful Algal Blooms.Environmental science & technology · 2025Article
- Health and Environmental Impacts of Cyanobacteria and Cyanotoxins from Freshwater to Seawater.Toxins · 2025Review
- Molecular Screening for Cyanobacteria and Their Cyanotoxin Potential in Diverse Habitats.Toxins · 2024Article
- The spatiotemporal distribution of potential saxitoxin-producing cyanobacteria in western Lake Erie.Journal of Great Lakes research · 2024Article
- Transdisciplinary approaches for the study of cyanobacteria and cyanotoxins.Current research in microbial sciences · 2024Article
- Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue.Toxics · 2023Article
- Potamoplankton of the Maumee River during 2018 and 2019: The relationship between cyanobacterial toxins and environmental factors.Journal of Great Lakes research · 2022Article
- Article
- Cyanotoxin-encoding genes as powerful predictors of cyanotoxin production during harmful cyanobacterial blooms in an inland freshwater lake: Evaluating a novel early-warning system.The Science of the total environment · 2022Article
- Article
- First Report on Microcystin-LR Occurrence in Water Reservoirs of Eastern Cuba, and Environmental Trigger Factors.Toxins · 2022Article
- Molecular and Pigment Analyses Provide Comparative Results When Reconstructing Historic Cyanobacterial Abundances from Lake Sediment Cores.Microorganisms · 2022Article
- Function-based classification of hazardous biological sequences: Demonstration of a new paradigm for biohazard assessments.Frontiers in bioengineering and biotechnology · 2022Article
- Depth-Dependent Spatiotemporal Dynamics of Overwintering PelagicMicroorganisms · 2021Article
- Molecular detection of some toxogenic cyanobacteria in Tigris River in Baghdad-Iraq.Molecular biology reports · 2021Article
- Potentially Toxic Planktic and Benthic Cyanobacteria in Slovenian Freshwater Bodies: Detection by Quantitative PCR.Toxins · 2021Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saxitoxins (STXs) are carbamate alkaloid neurotoxins produced by marine "red tide" dinoflagellates and several species of freshwater filamentous cyanobacteria, including Anabaena circinalis, Aphanizomenon spp., Lyngbya wollei, and Cylindrospermopsis raciborskii. A specific quantitative PCR (qPCR) method based on SYBR green chemistry was developed to quantify saxitoxin-producing Anabaena circinalis cyanobacteria, which are major bloom-forming freshwater cyanobacteria. The aim of this study was to infer the potential toxigenicity of samples by determining the copy number of a unique and unusual polyketide synthase (PKS) sequence (sxtA) in the STX biosynthesis gene cluster identified in cyanobacteria. Our qPCR approach was applied to water samples collected from different Australian lakes, dams, and rivers. The STX concentration and cyanobacterial cell density of these blooms were also determined by high-pressure liquid chromatography (HPLC) and microscopic cell counting, respectively. STX concentrations correlated positively with STX gene copy numbers, indicating that the latter can be used as a measure of potential toxigenicity in Anabaena circinalis and possibly other cyanobacterial blooms. The qPCR method targeting STX genes can also be employed for both monitoring and ecophysiological studies of toxic Anabaena circinalis blooms and potentially several other STX-producing cyanobacteria.
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