ArticleApplied and environmental microbiology2010
Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 102 citations in OpenAlex.
- Metabolic engineering strategies for sustainable de novo isobutylamine production by Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2026Article
- Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca.Microbial cell factories · 2024Article
- Strain improvement, artificial intelligence optimization, and sensitivity analysis of asparaginase-mediated acrylamide reduction in sweet potato chips.Journal of food science and technology · 2023Article
- System metabolic engineering ofFrontiers in bioengineering and biotechnology · 2023Article
- Metabolic engineering of Corynebacterium glutamicum for acetate-based itaconic acid production.Biotechnology for biofuels and bioproducts · 2022Article
- Isobutanol production by combinedMetabolic engineering communications · 2022Article
- Evaluation of Metabolic Engineering Strategies on 2-Ketoisovalerate Production by Escherichia coli.Applied and environmental microbiology · 2022Article
- Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production.World journal of microbiology & biotechnology · 2022Article
- Recombinant production of the lantibiotic nisin using Corynebacterium glutamicum in a two-step process.Microbial cell factories · 2022Article
- Establishing recombinant production of pediocin PA-1 in Corynebacterium glutamicum.Metabolic engineering · 2021Article
- Sustainable Production ofMicroorganisms · 2021Article
- The Industrial OrganismAntioxidants (Basel, Switzerland) · 2020Article
- Inducible Expression Systems Based on Xenogeneic Silencing and Counter-Silencing and Design of a Metabolic Toggle Switch.ACS synthetic biology · 2020Article
- EngineeringEngineering in life sciences · 2020Article
- Metabolic engineering ofSynthetic and systems biotechnology · 2019Article
- Modular systems metabolic engineering enables balancing of relevant pathways for l-histidine production withBiotechnology for biofuels · 2019Article
- Fermentative Production ofFrontiers in bioengineering and biotechnology · 2019Article
- Bromination of L-tryptophan in a Fermentative Process WithFrontiers in bioengineering and biotechnology · 2019Article
- One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst.Scientific reports · 2018Article
- Redirecting carbon flux through pgi-deficient and heterologous transhydrogenase toward efficient succinate production in Corynebacterium glutamicum.Journal of industrial microbiology & biotechnology · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2-Ketoisovalerate is used as a therapeutic agent, and a 2-ketoisovalerate-producing organism may serve as a platform for products deriving from this 2-keto acid. We engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the l-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production was further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 188 ± 28 mM (21.8 ± 3.2 g liter(-1)) 2-ketoisovalerate and showed a product yield of about 0.47 ± 0.05 mol per mol (0.3 ± 0.03 g per g) of glucose and a volumetric productivity of about 4.6 ± 0.6 mM (0.53 ± 0.07 g liter(-1)) 2-ketoisovalerate per h in the overall production phase. In studying the influence of the three branched-chain 2-keto acids 2-ketoisovalerate, 2-ketoisocaproate, and 2-keto-3-methylvalerate on the AHAS activity, we observed a competitive inhibition of the AHAS enzyme by 2-ketoisovalerate.
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