ArticleApplied and environmental microbiology2010
Escherichia coli binary culture engineered for direct fermentation of hemicellulose to a biofuel.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Data-driven synthetic microbes for sustainable future.NPJ systems biology and applications · 2025Review
- Spontaneously established syntrophic yeast communities improve bioproduction.Nature chemical biology · 2023Article
- Dynamic cybergenetic control of bacterial co-culture composition via optogenetic feedback.Nature communications · 2022Article
- Review
- Parsed synthesis of pyocyanin via co-culture enables context-dependent intercellular redox communication.Microbial cell factories · 2021Article
- Designing microbial communities to maximize the thermodynamic driving force for the production of chemicals.PLoS computational biology · 2021Article
- One-pot synthesis of 6-aminohexanoic acid from cyclohexane using mixed-species cultures.Microbial biotechnology · 2021Article
- A bottom-up approach towards a bacterial consortium for the biotechnological conversion of chitin to L-lysine.Applied microbiology and biotechnology · 2021Article
- Metabolic modelling approaches for describing and engineering microbial communities.Computational and structural biotechnology journal · 2021Review
- Deep reinforcement learning for the control of microbial co-cultures in bioreactors.PLoS computational biology · 2020Article
- Understanding the Mechanisms of Positive Microbial Interactions That Benefit Lactic Acid Bacteria Co-cultures.Frontiers in microbiology · 2020Review
- Bacterial co-culture with cell signaling translator and growth controller modules for autonomously regulated culture composition.Nature communications · 2019Article
- Simulations reveal challenges to artificial community selection and possible strategies for success.PLoS biology · 2019Article
- Recent advances of biofuels and biochemicals production from sustainable resources using co-cultivation systems.Biotechnology for biofuels · 2019Review
- Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane (Frontiers in plant science · 2019Article
- Tools for engineering coordinated system behaviour in synthetic microbial consortia.Nature communications · 2018Article
- Biorefining of protein waste for production of sustainable fuels and chemicals.Biotechnology for biofuels · 2018Review
- Heterologous biosynthesis of natural product naringenin by co-culture engineering.Synthetic and systems biotechnology · 2017Article
- Phenotypic and proteomic analysis of positively regulated gellan biosynthesis pathway inAnimal cells and systems · 2017Article
- Design, analysis and application of synthetic microbial consortia.Synthetic and systems biotechnology · 2016Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic engineering has created several Escherichia coli biocatalysts for production of biofuels and other useful molecules. However, the inability of these biocatalysts to directly use polymeric substrates necessitates costly pretreatment and enzymatic hydrolysis prior to fermentation. Consolidated bioprocessing has the potential to simplify the process by combining enzyme production, hydrolysis, and fermentation into a single step but requires a fermenting organism to multitask by producing both necessary enzymes and target molecules. We demonstrate here a binary strategy for consolidated bioprocessing of xylan, a complex substrate requiring six hemicellulases for complete hydrolysis. An integrated modular approach was used to design the two strains to function cooperatively in the process of transforming xylan into ethanol. The first strain was engineered to coexpress two hemicellulases. Recombinant enzymes were secreted to the growth medium by a method of lpp deletion with over 90% efficiency. Secreted enzymes hydrolyzed xylan into xylooligosaccharides, which were taken in by the second strain, designed to use the xylooligosaccharides for ethanol production. Cocultivation of the two strains converted xylan hemicellulose to ethanol with a yield about 55% of the theoretical value. Inclusion of other three hemicellulases improved the ethanol yield to 70%. Analysis of the culture broth showed that xylooligosaccharides with four or more xylose units were not utilized, suggesting that improving the use of higher xyloogligomers should be the focus in future efforts. This is the first demonstration of an engineered binary culture for consolidated bioprocessing of xylan. The modular design should allow the strategy to be adopted for a broad range of biofuel and biorefinery products.
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