ArticleNucleic acids research2011
Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 162 citations in OpenAlex.
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- A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice.ACS synthetic biology · 2023Article
- Targeted Integration of Transgenes at the Mouse Gt(ROSA)26Sor Locus.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Liver-expressedScience advances · 2020Article
- Improved Functionality of Exhausted Intrahepatic CXCR5+ CD8+ T Cells Contributes to Chronic Antigen Clearance Upon Immunomodulation.Frontiers in immunology · 2020Article
- Targeting cardiac fibrosis with engineered T cells.Nature · 2019Article
- Novel strategy for rapid functional in vivo validation of oncogenic drivers in haematological malignancies.Scientific reports · 2019Article
- Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.Cell · 2018Article
- Expansion of functional personalized cells with specific transgene combinations.Nature communications · 2018Article
- TLR9-Mediated Conditioning of Liver Environment Is Essential for Successful Intrahepatic Immunotherapy and Effective Memory Recall.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Controlled re-activation of epigenetically silenced Tet promoter-driven transgene expression by targeted demethylation.Nucleic acids research · 2017Article
- Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- The CpG-sites of the CBX3 ubiquitous chromatin opening element are critical structural determinants for the anti-silencing function.Scientific reports · 2017Article
- Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange.Journal of visualized experiments : JoVE · 2017Article
- High-fidelityMolecular metabolism · 2017Article
- CpG-ODN Facilitates Effective Intratracheal Immunization and Recall of Memory against Neoantigen-Expressing Alveolar Cells.Frontiers in immunology · 2017Article
- Effective intrahepatic CD8+ T-cell immune responses are induced by low but not high numbers of antigen-expressing hepatocytes.Cellular & molecular immunology · 2016Article
- p120 Catenin-Mediated Stabilization of E-Cadherin Is Essential for Primitive Endoderm Specification.PLoS genetics · 2016Article
- Recombinase-Mediated Cassette Exchange (RMCE)-in Reporter Cell Lines as an Alternative to the Flp-in System.PloS one · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant mouse strains that harbor tightly controlled transgene expression proved to be indispensible tools to elucidate gene function. Different strategies have been employed to achieve controlled induction of the transgene. However, many models are accompanied by a considerable level of basal expression in the non-induced state. Thereby, applications that request tight control of transgene expression, such as the expression of toxic genes and the investigation of immune response to neo antigens are excluded. We developed a new Cre/loxP-based strategy to achieve strict control of transgene expression. This strategy was combined with RMCE (recombinase mediated cassette exchange) that facilitates the targeting of genes into a tagged site in ES cells. The tightness of regulation was confirmed using luciferase as a reporter. The transgene was induced upon breeding these mice to effector animals harboring either the ubiquitous (ROSA26) or liver-specific (Albumin) expression of CreER(T2), and subsequent feeding with Tamoxifen. Making use of RMCE, luciferase was replaced by Ovalbumin antigen. Mice generated from these ES cells were mated with mice expressing liver-specific CreER(T2). The transgenic mice were examined for the establishment of an immune response. They were fully competent to establish an immune response upon hepatocyte specific OVA antigen expression as indicated by a massive liver damage upon Tamoxifen treatment and did not show OVA tolerance. Together, this proves that this strategy supports strict control of transgenes that is even compatible with highly sensitive biological readouts.
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