ArticleJournal of the American Chemical Society2010
Probing the spontaneous membrane insertion of a tail-anchored membrane protein by sum frequency generation spectroscopy.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Otoferlin C2F Domain-Induced Changes in Membrane Structure Observed by Sum Frequency Generation.Biophysical journal · 2019Article
- Nonlinear Optical Methods for Characterization of Molecular Structure and Surface Chemistry.Topics in catalysis · 2018Article
- In Situ Investigation of Peptide-Lipid Interaction Between PAP248-286 and Model Cell Membranes.The Journal of membrane biology · 2016Article
- Evidence of a molecular boundary lubricant at snakeskin surfaces.Journal of the Royal Society, Interface · 2015Article
- Diatom mimics: directing the formation of biosilica nanoparticles by controlled folding of lysine-leucine peptides.Journal of the American Chemical Society · 2014Article
- ¹H, ¹³C and ¹⁵N resonance assignments for the full-length mammalian cytochrome b₅ in a membrane environment.Biomolecular NMR assignments · 2014Article
- Probing the orientation of electrostatically immobilized cytochrome C by time of flight secondary ion mass spectrometry and sum frequency generation spectroscopy.Biointerphases · 2013Article
- Site-specific orientation of an α-helical peptide ovispirin-1 from isotope-labeled SFG spectroscopy.The journal of physical chemistry. B · 2013Article
- Lipid Fluid-Gel Phase Transition Induced Alamethicin Orientational Change Probed by Sum Frequency Generation Vibrational Spectroscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2013Article
- A model of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data.The Journal of biological chemistry · 2013Article
- Membrane orientation of Gα(i)β(1)γ(2) and Gβ(1)γ(2) determined via combined vibrational spectroscopic studies.Journal of the American Chemical Society · 2013Article
- Dependence of Alamethicin Membrane Orientation on the Solution Concentration.The journal of physical chemistry. C, Nanomaterials and interfaces · 2013Article
- Protein-phospholipid interactions in nonclassical protein secretion: problem and methods of study.International journal of molecular sciences · 2013Article
- Physiologically-relevant modes of membrane interactions by the human antimicrobial peptide, LL-37, revealed by SFG experiments.Scientific reports · 2013Article
- Elucidation of molecular structures at buried polymer interfaces and biological interfaces using sum frequency generation vibrational spectroscopy.Soft matter · 2013Article
- Interkingdom signaling: integration, conformation, and orientation of N-acyl-L-homoserine lactones in supported lipid bilayers.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Observing a model ion channel gating action in model cell membranes in real time in situ: membrane potential change induced alamethicin orientation change.Journal of the American Chemical Society · 2012Article
- Molecular interactions between cell penetrating peptide Pep-1 and model cell membranes.The journal of physical chemistry. B · 2012Article
- Probing the orientation of electrostatically immobilized Protein G B1 by time-of-flight secondary ion spectrometry, sum frequency generation, and near-edge X-ray adsorption fine structure spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Heterotrimeric G protein beta1gamma2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In addition to providing a semipermeable barrier that protects a cell from harmful stimuli, lipid membranes occupy a central role in hosting a variety of biological processes, including cellular communications and membrane protein functions. Most importantly, protein-membrane interactions are implicated in a variety of diseases and therefore many analytical techniques were developed to study the basis of these interactions and their influence on the molecular architecture of the cell membrane. In this study, sum frequency generation (SFG) vibrational spectroscopy is used to investigate the spontaneous membrane insertion process of cytochrome b(5) and its mutants. Experimental results show a significant difference in the membrane insertion and orientation properties of these proteins, which can be correlated with their functional differences. In particular, our results correlate the nonfunctional property of a mutant cytochrome b(5) with its inability to insert into the lipid bilayer. The approach reported in this study could be used as a potential rapid screening tool in measuring the topology of membrane proteins as well as interactions of biomolecules with lipid bilayers in situ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.