ArticleApplied microbiology and biotechnology2010
Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon.
Article in Applied microbiology and biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Does the microbiome play a role in the pathogenesis of colonic diverticular disease? A systematic review.Journal of gastroenterology and hepatology · 2023Pooled it
- In Situ Laser-Capture Microdissection for Detection of Components of the Hair Follicle and Scalp Microbiome.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Isolation of mucosa-associated microbiota dysbiosis in the ascending colon in hepatitis C virus post-sustained virologic response cirrhotic patients.Frontiers in cellular and infection microbiology · 2024Article
- Atypical behavioral and thermoregulatory circadian rhythms in mice lacking a microbiome.Scientific reports · 2022Article
- A pilot study demonstrating the impact of surgical bowel preparation on intestinal microbiota composition following colon and rectal surgery.Scientific reports · 2022Article
- Spatial distribution of live gut microbiota and bile acid metabolism in various parts of human large intestine.Scientific reports · 2022Article
- Wolf in Sheep's Clothing:Microorganisms · 2021Review
- Host/microbiota interactions in health and diseases-Time for mucosal microbiology!Mucosal immunology · 2021Review
- Technological tools and strategies for culturing human gut microbiota in engineered in vitro models.Biotechnology and bioengineering · 2021Review
- Gastrointestinal biofilms in health and disease.Nature reviews. Gastroenterology & hepatology · 2021Review
- Article
- Identification of Inner Mucus-Associated Bacteria by Laser Capture Microdissection.Cellular and molecular gastroenterology and hepatology · 2019Article
- Microbiota and metabolism: what's new in 2018?American journal of physiology. Endocrinology and metabolism · 2018Review
- Microbiota profile in new-onset pediatric Crohn's disease: data from a non-Western population.Gut pathogens · 2018Article
- De Novo Synthesis and Functional Analysis of Polyphosphate-Loaded Poly(Ethylene) Glycol Hydrogel Nanoparticles Targeting Pyocyanin and Pyoverdin Production in Pseudomonas aeruginosa as a Model Intestinal Pathogen.Annals of biomedical engineering · 2017Article
- Can We Prevent Obesity-Related Metabolic Diseases by Dietary Modulation of the Gut Microbiota?Advances in nutrition (Bethesda, Md.) · 2016Review
- Article
- Micromanagement in the gut: microenvironmental factors govern colon mucosal biofilm structure and functionality.NPJ biofilms and microbiomes · 2015Review
- Intestinal anastomotic injury alters spatially defined microbiome composition and function.Microbiome · 2014Article
- Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model.The ISME journal · 2013Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Metagenomic analysis of colonic mucosa-associated microbes has been complicated by technical challenges that disrupt or alter community structure and function. In the present study, we determined the feasibility of laser capture microdissection (LCM) of intact regional human colonic mucosa-associated microbes followed by phi29 multiple displacement amplification (MDA) and massively parallel sequencing for metagenomic analysis. Samples were obtained from the healthy human subject without bowel preparation and frozen sections immediately prepared. Regional mucosa-associated microbes were successfully dissected using LCM with minimal contamination by host cells, their DNA extracted and subjected to phi29 MDA with a high fidelity, prior to shotgun sequencing using the GS-FLX DNA sequencer. Metagenomic analysis of approximately 67 million base pairs of DNA sequences from two samples revealed that the metabolic functional profiles in mucosa-associated microbes were as diverse as those reported in feces, specifically the representation of functional genes associated with carbohydrate, protein, and nucleic acid utilization. In summary, these studies demonstrate the feasibility of the approach to study the structure and metagenomic profiles of human intestinal mucosa-associated microbial communities at small spatial scales.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.