ArticleWorld journal of microbiology & biotechnology2010
An efficient gene deletion procedure for the mushroom-forming basidiomycete Schizophyllum commune.
Article in World journal of microbiology & biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 59 citations in OpenAlex.
- Functions of the cell wall polysaccharide schizophyllan during vegetative growth ofCell surface (Amsterdam, Netherlands) · 2026Article
- Targeted insertion of heterogenous DNA using Cas9-gRNA ribonucleoprotein-mediated gene editing inBioengineered · 2025Article
- Strongest untreated mycelium materials produced by Schizophyllum commune dikaryons.World journal of microbiology & biotechnology · 2025Article
- CRISPR/Cas9-mediated genome editing in Ganoderma lucidum: recent advances and biotechnological opportunities.World journal of microbiology & biotechnology · 2025Review
- Comparative transcriptome analyses and CRISPR/Cas9-mediated functional study ofFrontiers in microbiology · 2025Article
- Effect of a Mating Type Gene Editing inJournal of fungi (Basel, Switzerland) · 2024Article
- The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments.Scientific reports · 2023Article
- Preassembled Cas9 Ribonucleoprotein-Mediated Gene Deletion Identifies the Carbon Catabolite Repressor and Its Target Genes in Coprinopsis cinerea.Applied and environmental microbiology · 2022Article
- Article
- H3K4me2 ChIP-Seq reveals the epigenetic landscape during mushroom formation and novel developmental regulators of Schizophyllum commune.Scientific reports · 2021Article
- Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research.Biology open · 2020Review
- Fungal evolution: cellular, genomic and metabolic complexity.Biological reviews of the Cambridge Philosophical Society · 2020Article
- Interruption of an MSH4 homolog blocks meiosis in metaphase I and eliminates spore formation in Pleurotus ostreatus.PloS one · 2020Article
- High-throughput targeted gene deletion in the model mushroom Schizophyllum commune using pre-assembled Cas9 ribonucleoproteins.Scientific reports · 2019Article
- Schizophyllum commune: An unexploited source for lignocellulose degrading enzymes.MicrobiologyOpen · 2018Review
- Nucleus-specific expression in the multinuclear mushroom-forming fungusProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth.Scientific reports · 2017Article
- Schizophyllum commune has an extensive and functional alternative splicing repertoire.Scientific reports · 2016Article
- The good, the bad and the tasty: The many roles of mushrooms.Studies in mycology · 2016Article
- Xenomic networks variability and adaptation traits in wood decaying fungi.Microbial biotechnology · 2013Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene deletion in Schizophyllum commune is hampered by a low incidence of homologous integration. As a consequence, extensive screening is required to identify a transformant with the desired genotype. To alleviate this and to facilitate the assembly of deletion plasmids, vector pDelcas was constructed. This construct has a set of restriction sites, which allows for directional cloning of the flanking sequences at both sides of a nourseothricin resistance cassette. Moreover, it contains a phleomycin resistance cassette elsewhere in the plasmid, which is used to screen for transformants with an ectopic integration of the pDelcas derivative. The use of pDelcas derivatives in combination with an improved PCR screening protocol permitted the efficient identification of S. commune deletion strains. This procedure may also function in other basidiomycetes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11274-010-0356-0) contains supplementary material, which is available to authorized users.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.