ArticleNucleic acids research2011
Amplification of a plasmid bearing a mammalian replication initiation region in chromosomal and extrachromosomal contexts.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Targeted amplification of a sequence of interest in artificial chromosome in mammalian cells.Nucleic acids research · 2019Article
- Generation and maintenance of acentric stable double minutes from chromosome arms in inter-species hybrid cells.BMC molecular and cell biology · 2019Article
- Complex repeat structure promotes hyper-amplification and amplicon evolution through rolling-circle replication.Nucleic acids research · 2018Article
- Reduced cohesin destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability.Nucleic acids research · 2016Article
- Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells.PloS one · 2016Article
- Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing.PloS one · 2016Article
- Genomic organization and evolution of double minutes/homogeneously staining regions with MYC amplification in human cancer.Nucleic acids research · 2014Article
- How a replication origin and matrix attachment region accelerate gene amplification under replication stress in mammalian cells.PloS one · 2014Article
- Two replication fork maintenance pathways fuse inverted repeats to rearrange chromosomes.Nature · 2013Article
- Article
- Article
- Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method.PloS one · 2012Article
- Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification.Nucleic acids research · 2011Article
- Generation of micronuclei during interphase by coupling between cytoplasmic membrane blebbing and nuclear budding.PloS one · 2011Article
- Application of the novel and convenient IR/MAR gene amplification technology to the production of recombinant protein pharmaceuticals.BMC proceedings · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amplified genes in cancer cells reside on extrachromosomal double minutes (DMs) or chromosomal homogeneously staining regions (HSRs). We used a plasmid bearing a mammalian replication initiation region to model gene amplification. Recombination junctions in the amplified region were comprehensively identified and sequenced. The junctions consisted of truncated direct repeats (type 1) or inverted repeats (type 2) with or without spacing. All of these junctions were frequently detected in HSRs, whereas there were few type 1 or a unique type 2 flanked by a short inverted repeat in DMs. The junction sequences suggested a model in which the inverted repeats were generated by sister chromatid fusion. We were consistently able to detect anaphase chromatin bridges connected by the plasmid repeat, which were severed in the middle during mitosis. De novo HSR generation was observed in live cells, and each HSR was lengthened more rapidly than expected from the classical breakage/fusion/bridge model. Importantly, we found massive DNA synthesis at the broken anaphase bridge during the G1 to S phase, which could explain the rapid lengthening of the HSR. This mechanism may not operate in acentric DMs, where most of the junctions are eliminated and only those junctions produced through stable intermediates remain.
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