ArticleBMC genomics2010
Unique genome-wide transcriptome profiles of chicken macrophages exposed to Salmonella-derived endotoxin.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 73 citations in OpenAlex.
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- Heat Stress Influences Immunity ThroughAnimals : an open access journal from MDPI · 2025Article
- Length-Dependent Modulation of B Cell Activating Factor Transcripts in Chicken Macrophage by Viral Double-Stranded RNA.Vaccines · 2023Article
- Dual RNA-Seq transcriptome analysis of chicken macrophage-like cells (HD11) infectedParasitology · 2021Article
- Dual Transcriptomic Analyses Unveil Host-Pathogen Interactions BetweenFrontiers in microbiology · 2021Article
- Chicken cecal DNA methylome alteration in the response to Salmonella enterica serovar Enteritidis inoculation.BMC genomics · 2020Article
- The Differential Phosphorylation-Dependent Signaling and Glucose Immunometabolic Responses Induced during Infection byMicroorganisms · 2020Article
- Concordant expression pattern across multiple immune tissues of commercial broilers in response to avian pathogenic3 Biotech · 2019Article
- Emerging Roles for NLRC5 in Immune Diseases.Frontiers in pharmacology · 2019Review
- Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses.PloS one · 2019Article
- Comparative roles of clpA and clpB in the survival of S. Typhimurium under stress and virulence in poultry.Scientific reports · 2018Article
- Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer.BMC genomics · 2017Article
- Heat Stress and Lipopolysaccharide Stimulation of Chicken Macrophage-Like Cell Line Activates Expression of Distinct Sets of Genes.PloS one · 2016Article
- Avian pathogenic Escherichia coli (APEC) infection alters bone marrow transcriptome in chickens.BMC genomics · 2015Article
- Characterization of a novel chicken muscle disorder through differential gene expression and pathway analysis using RNA-sequencing.BMC genomics · 2015Article
- Differential modulation of immune response and cytokine profiles in the bursae and spleen of chickens infected with very virulent infectious bursal disease virus.BMC veterinary research · 2015Article
- Cell-autonomous sex differences in gene expression in chicken bone marrow-derived macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Functional Analysis of Chicken IRF7 in Response to dsRNA Analog Poly(I:C) by Integrating Overexpression and Knockdown.PloS one · 2015Article
- RNA-seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature.BMC genomics · 2014Article
- DNA methylome in spleen of avian pathogenic Escherichia coli-challenged broilers and integration with mRNA expression.Scientific reports · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMacrophages play essential roles in both innate and adaptive immune responses. Bacteria require endotoxin, a complex lipopolysaccharide, for outer membrane permeability and the host interprets endotoxin as a signal to initiate an innate immune response. The focus of this study is kinetic and global transcriptional analysis of the chicken macrophage response to in vitro stimulation with endotoxin from Salmonella typhimurium-798.
resultsThe 38535-probeset Affymetrix GeneChip Chicken Genome array was used to profile transcriptional response to endotoxin 1, 2, 4, and 8 hours post stimulation (hps). Using a maximum FDR (False Discovery Rate) of 0.05 to declare genes as differentially expressed (DE), we found 13, 33, 1761 and 61 DE genes between endotoxin-stimulated versus non-stimulated cells at 1, 2, 4 and 8 hps, respectively. QPCR demonstrated that endotoxin exposure significantly affected the mRNA expression of IL1B, IL6, IL8, and TLR15, but not IL10 and IFNG in HD 11 cells. Ingenuity Pathway Analysis showed that 10% of the total DE genes were involved in inflammatory response. Three, 9.7, 96.8, and 11.8% of the total DE inflammatory response genes were significantly differentially expressed with endotoxin stimulation at 1, 2, 4 and 8 hps, respectively. The NFKBIA, IL1B, IL8 and CCL4 genes were consistently induced at all times after endotoxin treatment. NLRC5 (CARD domain containing, NOD-like receptor family, RCJMB04_18i2), an intracellular receptor, was induced in HD11 cells treated with endotoxin.
conclusionsAs above using an in vitro model of chicken response to endotoxin, our data revealed the kinetics of gene networks involved in host response to endotoxin and extend the known complexity of networks in chicken immune response to Gram-negative bacteria such as Salmonella. The induction of NFKBIA, IL1B, IL8, CCL4 genes is a consistent signature of host response to endotoxin over time. We make the first report of induction of a NOD-like receptor family member in response to Salmonella endotoxin in chicken macrophages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.