ArticleMicrobial ecology2011
Investigation of supragingival plaque microbiota in different caries status of Chinese preschool children by denaturing gradient gel electrophoresis.
Article in Microbial ecology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Trial
- Ancient oral microbiomes support gradual Neolithic dietary shifts towards agriculture.Nature communications · 2022Article
- Expert consensus on early childhood caries management.International journal of oral science · 2022Review
- Cariogenic and oral health taxa in the oral cavity among children and adults: A scoping review.Archives of oral biology · 2021Article
- High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.Journal of Zhejiang University. Science. B · 2021Article
- Analysis of oral microbial dysbiosis associated with early childhood caries.BMC oral health · 2021Article
- [Recent achievements in the microbiological etiology of dental caries].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2018Article
- Effect of SrtA on Interspecies Adherence of Oral Bacteria.Current medical science · 2018Article
- Review
- [Bacterial diversity in the oral cavity of adolescents with different caries susceptibilities].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2015Article
- Urinary microbiota in patients with prostate cancer and benign prostatic hyperplasia.Archives of medical science : AMS · 2015Article
- The impact of various time intervals on the supragingival plaque dynamic core microbiome.PloS one · 2015Article
- Frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity.Dental research journal · 2013Article
- Topical effect of a medically prescribed pediatric antibiotic on dental biofilm: a cross-over, in situ study.PloS one · 2013Article
- Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation.Asian journal of andrology · 2012Article
- Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis.PloS one · 2012Article
- Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis.BMC oral health · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to detect differences in the richness of total supragingival plaque microbiota as well as the species composition of oral streptococci involved in the different stages of dental caries. Forty-five plaque samples were collected from caries-moderate (CM, 4 ≤ dmfs ≤ 6), caries-susceptible (CS, dmfs ≥ 10), and age-matched caries-free children separately. Total DNA was isolated directly from each sample, and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analyses using universal and primers specific for oral streptococci were carried out. Using 16S rDNA PCR-DGGE, 34 different species of bacteria were identified in a culture-independent manner and classified into 11 genera according to phylogenetic analysis. Among them, Mitis group streptococci and Campylobacter, which were present in health status, no longer appeared in caries-susceptible samples. In addition, Capnocytophaga, Burkholderia, and Prevotella were found significantly less frequently in the CS group samples (P < 0.05), while there were no significant differences among the prevalence of Neisseria, Leptotrichia, Haemophilus, Mutans group streptococci, Corynebacterium, and Actinomyces in the three groups. Further DGGE analysis of rnpB gene amplicons obtained with oral streptococci species-specific primers showed that a total of 23 species of oral streptococci were identified. Streptococcus sanguinis, Streptococcus mitis, and Streptococcus oralis showed a significantly higher prevalence in healthy children (P < 0.05), while that of Streptococcus mutans and Streptococcus sobrinus did not vary among the three groups. Overall, these results suggest that supragingival plaque microbiota as a whole undergoes a more complicated shift in the caries process.
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Registered trials
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