ArticleLeukemia2010
A DEAB-sensitive aldehyde dehydrogenase regulates hematopoietic stem and progenitor cells development during primitive hematopoiesis in zebrafish embryos.
Article in Leukemia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Zebrafish as a Model to Study Retinoic Acid Signaling in Development and Disease.Biomedicines · 2023Review
- Aldh2 is a lineage-specific metabolic gatekeeper in melanocyte stem cells.Development (Cambridge, England) · 2022Article
- Making Blood from the Vessel: Extrinsic and Environmental Cues Guiding the Endothelial-to-Hematopoietic Transition.Life (Basel, Switzerland) · 2021Review
- Alcohol abuse and disorder of granulopoiesis.Pharmacology & therapeutics · 2019Review
- The zebrafish: A fintastic model for hematopoietic development and disease.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Genetic Models of Leukemia in Zebrafish.Frontiers in cell and developmental biology · 2018Review
- Pathways of retinoid synthesis in mouse macrophages and bone marrow cells.Journal of leukocyte biology · 2016Article
- Zebrafish retinal defects induced by ethanol exposure are rescued by retinoic acid and folic acid supplement.Alcohol (Fayetteville, N.Y.) · 2015Article
- Absence of natural intracellular retinoids in mouse bone marrow cells and implications for PML-RARA transformation.Blood cancer journal · 2015Article
- Interaction of infectious spleen and kidney necrosis virus ORF119L with PINCH leads to dominant-negative inhibition of integrin-linked kinase and cardiovascular defects in zebrafish.Journal of virology · 2015Article
- RA and FGF signalling are required in the zebrafish otic vesicle to pattern and maintain ventral otic identities.PLoS genetics · 2014Article
- Aldehyde dehydrogenases: from eye crystallins to metabolic disease and cancer stem cells.Chemico-biological interactions · 2013Review
- Genome-wide association analysis of Sasang constitution in the Korean population.Journal of alternative and complementary medicine (New York, N.Y.) · 2012Article
- A zebrafish model of axenfeld-rieger syndrome reveals that pitx2 regulation by retinoic acid is essential for ocular and craniofacial development.Investigative ophthalmology & visual science · 2012Article
- Retinoic acid signaling plays a restrictive role in zebrafish primitive myelopoiesis.PloS one · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although aldehyde dehydrogenase (ALDH) activity has become a surrogate of hematopoietic stem and progenitor cells (HSPCs), its function during hematopoiesis was unclear. Here, we examined its role in zebrafish hematopoiesis based on pharmacological inhibition and morpholino (MO) knockdown. Zebrafish embryos were treated with diethylaminobenzaldehyde (DEAB, 1 μmol/l) between 0- and 48 hour-post-fertilization (hpf). MOs targeting aldhs were injected between 1 and 4-cell stage. The effects on hematopoiesis were evaluated at different stages. DEAB treatment between 0 and 18 hpf increased gene expression associated with HSPC (scl, lmo2), erythropoiesis (gata1, α- and β-eHb) and myelopoiesis (spi1) as well as gfp(+) cells in dissociated Tg(gata1:gfp) embryos. The effects were ameliorated by all-trans retinoic acid (1 nmol/l). Definitive hematopoiesis and the erythromyeloid precursors were unaffected. In all, 14 out of 15 zebrafish aldhs were detectable by reverse transcription PCR in 18 hpf embryos, of which only aldh1a2 and aldh16a1 were expressed in sites pertinent to hematopoiesis. Molecular targeting by MOs was demonstrated for 15 aldhs, but none of them, even in combined aldh1a2 and aldh1a3 knockdown, recapitulated the hematopoietic expansion in DEAB-treated embryos. In conclusion, DEAB expands HSPC population during primitive hematopoiesis through inhibition of aldh and retinoic acid synthesis. The specific aldh isoform(s) remains to be determined.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.