ArticleKidney international2011
Angiotensin II diminishes the effect of SGK1 on the WNK4-mediated inhibition of ROMK1 channels.
Article in Kidney international, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- The Story of Aldosterone Escape.Journal of the American Society of Nephrology : JASN · 2026Review
- Angiotensin II hypertension along the female rat tubule: predicted impact on coupled transport of NaAmerican journal of physiology. Renal physiology · 2023Article
- Mechanisms coupling sodium and magnesium reabsorption in the distal convoluted tubule of the kidney.Acta physiologica (Oxford, England) · 2021Review
- The Angiotensin II Type 1 Receptor-Associated Protein Attenuates Angiotensin II-Mediated Inhibition of the Renal Outer Medullary Potassium Channel in Collecting Duct Cells.Frontiers in physiology · 2021Article
- Angiotensin II Signal Transduction: An Update on Mechanisms of Physiology and Pathophysiology.Physiological reviews · 2018Review
- Potassium and Its Discontents: New Insight, New Treatments.Journal of the American Society of Nephrology : JASN · 2016Review
- Article
- Changes in urinary excretion of water and sodium transporters during amiloride and bendroflumethiazide treatment.World journal of nephrology · 2015Article
- Regulation of Potassium Homeostasis.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- Dietary potassium and the renal control of salt balance and blood pressure.Pflugers Archiv : European journal of physiology · 2015Review
- The sodium chloride cotransporter SLC12A3: new roles in sodium, potassium, and blood pressure regulation.Pflugers Archiv : European journal of physiology · 2014Review
- Renal proteinase-activated receptor 2, a new actor in the control of blood pressure and plasma potassium level.The Journal of biological chemistry · 2013Article
- Direct activation of ENaC by angiotensin II: recent advances and new insights.Current hypertension reports · 2013Review
- Key developments in renin-angiotensin-aldosterone system inhibition.Nature reviews. Nephrology · 2013Review
- Regulation of the renal Na+-Cl- cotransporter by phosphorylation and ubiquitylation.American journal of physiology. Renal physiology · 2012Review
- Protein phosphatase 1 modulates the inhibitory effect of With-no-Lysine kinase 4 on ROMK channels.American journal of physiology. Renal physiology · 2012Article
- Aldosterone does not require angiotensin II to activate NCC through a WNK4-SPAK-dependent pathway.Pflugers Archiv : European journal of physiology · 2012Article
- Activation of the renal Na+:Cl- cotransporter by angiotensin II is a WNK4-dependent process.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- WNK kinases and the kidney.Experimental cell research · 2012Review
- Regulation of transport in the connecting tubule and cortical collecting duct.Comprehensive Physiology · 2012Review
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6 authors at 1 institution in 1 country.
Funding
Abstract
ROMK1 channels are located in the apical membrane of the connecting tubule and cortical collecting duct and mediate the potassium secretion during normal dietary intake. We used a perforated whole-cell patch clamp to explore the effect of angiotensin II on these channels in HEK293 cells transfected with green fluorescent protein (GFP)-ROMK1. Angiotensin II inhibited ROMK1 channels in a dose-dependent manner, an effect abolished by losartan or by inhibition of protein kinase C. Furthermore, angiotensin II stimulated a protein kinase C-sensitive phosphorylation of tyrosine 416 within c-Src. Inhibition of protein tyrosine kinase attenuated the effect of angiotensin II. Western blot studies suggested that angiotensin II inhibited ROMK1 channels by enhancing its tyrosine phosphorylation, a notion supported by angiotensin II's failure to inhibit potassium channels in cells transfected with the ROMK1 tyrosine mutant (R1Y337A). However, angiotensin II restored the with-no-lysine kinase-4 (WNK4)-induced inhibition of R1Y337A in the presence of serum-glucocorticoids-induced kinase 1 (SGK1), which reversed the inhibitory effect of WNK4 on ROMK1. Moreover, protein tyrosine kinase inhibition abolished the angiotensin II-induced restoration of WNK4-mediated inhibition of ROMK1. Angiotensin II inhibited ROMK channels in the cortical collecting duct of rats on a low sodium diet, an effect blocked by protein tyrosine kinase inhibition. Thus, angiotensin II inhibits ROMK channels by two mechanisms: increasing tyrosine phosphorylation of the channel and synergizing the WNK4-induced inhibition. Hence, angiotensin II may have an important role in suppressing potassium secretion during volume depletion.
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