ArticleAmerican journal of physiology. Cell physiology2010
Pathways for ATP release by bovine ciliary epithelial cells, the initial step in purinergic regulation of aqueous humor inflow.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Topical Sustained-Release Dexamethasone-Loaded Chitosan Nanoparticles: Assessment of Drug Delivery Efficiency in a Rabbit Model of Endotoxin-Induced Uveitis.Pharmaceutics · 2023Article
- The ocular pulse decreases aqueous humor outflow resistance by stimulating nitric oxide production.American journal of physiology. Cell physiology · 2021Article
- Potential Involvement of Adiponectin Signaling in Regulating Physical Exercise-Elicited Hippocampal Neurogenesis and Dendritic Morphology in Stressed Mice.Frontiers in cellular neuroscience · 2020Article
- Article
- Cell culture: complications due to mechanical release of ATP and activation of purinoceptors.Cell and tissue research · 2017Review
- Hyperosmotic stress induces ATP release and changes in P2X7 receptor levels in human corneal and conjunctival epithelial cells.Purinergic signalling · 2017Article
- The Role of the P2X7 Receptor in Ocular Stresses: A Potential Therapeutic Target.Vision (Basel, Switzerland) · 2017Review
- The Significance of TRPV4 Channels and Hemichannels in the Lens and Ciliary Epithelium.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2016Review
- ATP release through pannexon channels.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Rat, mouse, and primate models of chronic glaucoma show sustained elevation of extracellular ATP and altered purinergic signaling in the posterior eye.Investigative ophthalmology & visual science · 2015Article
- The role of activated adenosine receptors in degranulation of human LAD2 mast cells.Purinergic signalling · 2014Article
- Digoxin derivatives with enhanced selectivity for the α2 isoform of Na,K-ATPase: effects on intraocular pressure in rabbits.The Journal of biological chemistry · 2014Article
- The pannexins: past and present.Frontiers in physiology · 2014Review
- Regulation of cellular communication by signaling microdomains in the blood vessel wall.Pharmacological reviews · 2014Review
- Emerging functions of pannexin 1 in the eye.Frontiers in cellular neuroscience · 2014Review
- The progressive ankylosis gene product ANK regulates extracellular ATP levels in primary articular chondrocytes.Arthritis research & therapy · 2013Article
- CALHM1 ion channel mediates purinergic neurotransmission of sweet, bitter and umami tastes.Nature · 2013Article
- Physiological mechanisms for the modulation of pannexin 1 channel activity.The Journal of physiology · 2012Review
- Vesicular and conductive mechanisms of nucleotide release.Purinergic signalling · 2012Review
- Intercellular Ca(2+) waves: mechanisms and function.Physiological reviews · 2012Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
ATP release by nonpigmented (NPE) and pigmented (PE) ciliary epithelial cells is the enabling step in purinergic regulation of aqueous humor formation, but the release pathways are unknown. We measured ATP release from primary cultures of bovine mixed NPE and PE (bCE) cells and transformed bovine NPE and PE cells, using the luciferin-luciferase reaction. Hypotonicity-triggered bCE ATP release was inhibited by the relatively selective blocker of pannexin-1 (PX1) hemichannels (probenecid, 1 mM, 47 ± 2%), by a connexin inhibitor (heptanol, 1 mM, 49 ± 4%), and by an inhibitor of vesicular release (bafilomycin A1, 25 ± 2%), but not by the P2X(7) receptor (P2RX(7)) antagonist KN-62. Bafilomycin A1 acts by reducing the driving force for uptake of ATP from the cytosol into vesicles. The reducing agent dithiothreitol reduced probenecid-blockable ATP release. Similar results were obtained with NPE and PE cell lines. Pannexins PX1-3, connexins Cx43 and Cx40, and P2RX(7) were identified in native cells and cell lines by RT-PCR. PX1 mRNA expression was confirmed by Northern blots; its quantitative expression was comparable to that of Cx43 by real-time PCR. Heterologous expression of bovine PX1 in HEK293T cells enhanced swelling-activated ATP release, inhibitable by probenecid. We conclude that P2RX(7)-independent PX1 hemichannels, Cx hemichannels, and vesicular release contribute comparably to swelling-triggered ATP release. The relatively large response to dithiothreitol raises the possibility that the oxidation-reduction state is a substantial regulator of PX1-mediated ATP release from bovine ciliary epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.