ArticleSmall (Weinheim an der Bergstrasse, Germany)2010
An intein-mediated site-specific click conjugation strategy for improved tumor targeting of nanoparticle systems.
Article in Small (Weinheim an der Bergstrasse, Germany), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 62 citations in OpenAlex.
- Multi-epitope ligand-conjugated nanoparticles for tumor neoantigen targeting: advancing molecular precision in cancer immunotherapy.Medical oncology (Northwood, London, England) · 2025Review
- Research Progress on Cyclic-Peptide Functionalized Nanoparticles for Tumor-Penetrating Delivery.International journal of nanomedicine · 2024Review
- MNPs-IHSPN nanoparticles in multi-application with absorption of bio drugs in vitro.Biochemistry and biophysics reports · 2021Article
- Comparative Evaluation of Engineered Polypeptide Scaffolds in HER2-Targeting Magnetic Nanocarrier Delivery.ACS omega · 2021Article
- Impacting Pancreatic Cancer Therapy in HeterotypicNano letters · 2019Article
- Advances in Receptor-Mediated, Tumor-Targeted Drug Delivery.Advanced therapeutics · 2019Article
- Site-Specific Labeling of Cyanine and Porphyrin Dye-Stabilized Nanoemulsions with Affibodies for Cellular Targeting.Journal of the American Chemical Society · 2018Article
- Site-Specific Antibody Functionalization Using Tetrazine-Styrene Cycloaddition.Bioconjugate chemistry · 2018Article
- Forming next-generation antibody-nanoparticle conjugates through the oriented installation of non-engineered antibody fragments.Chemical science · 2018Article
- Advances in nano-based inner ear delivery systems for the treatment of sensorineural hearing loss.Advanced drug delivery reviews · 2017Review
- In Vivo HER2-Targeted Magnetic Resonance Tumor Imaging Using Iron Oxide Nanoparticles Conjugated with Anti-HER2 Fragment Antibody.Molecular imaging and biology · 2016Article
- Recent advances in covalent, site-specific protein immobilization.F1000Research · 2016Review
- Click Chemistry-Mediated Nanosensors for Biochemical Assays.Theranostics · 2016Review
- Recent advances in click chemistry applied to dendrimer synthesis.Molecules (Basel, Switzerland) · 2015Review
- An evolved Mxe GyrA intein for enhanced production of fusion proteins.ACS chemical biology · 2015Article
- A novel chitosan-hydrogel-based nanoparticle delivery system for local inner ear application.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2015Article
- Nanogel Carrier Design for Targeted Drug Delivery.Journal of materials chemistry. B · 2014Article
- Optimization of photoactive protein Z for fast and efficient site-specific conjugation of native IgG.Bioconjugate chemistry · 2014Article
- Facile method for the site-specific, covalent attachment of full-length IgG onto nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2014Article
- Quantitative comparison of tumor delivery for multiple targeted nanoparticles simultaneously by multiplex ICP-MS.Scientific reports · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to modify and directly target nanoparticulate carriers has greatly increased their applicability in diagnostic and therapeutic studies. Generally essential to the targeting of nanoparticles is the bioconjugation of targeting ligands to the agent's surface. While bioconjugation techniques have steadily improved in recent years, the field is still plagued with inefficient conjugations reactions and/or the lack of site-specific coupling. To overcome these limitations, click chemistry and expressed protein ligation (EPL) are combined to produce a highly efficient, site-specific reaction. This new EPL-click conjugation strategy is applied to create superparamagnetic iron oxide nanoparticles (SPIO) labeled with HER2/neu affibodies. These HER2-SPIO nanoparticles prove to be highly potent and receptor-specific in both in vitro cell studies and murine tumor models. Moreover, when EPL-click-derived HER2-SPIO are compared with SPIO that had been labeled with HER2 affibodies using other popular bioconjugation methods, they produce a statistically significant improvement in contrast enhancement upon cell binding. The EPL-click system is also successfully extended to other nanoparticle platforms (i.e., liposomes and dendrimers) highlighting the versatility of the approach.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.