ArticleThe Journal of biological chemistry2010
Fission yeast Swi1-Swi3 complex facilitates DNA binding of Mrc1.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Article
- Diploidy confers genomic instability inbioRxiv : the preprint server for biology · 2025Article
- The Fork Protection Complex: A Regulatory Hub at the Head of the Replisome.Sub-cellular biochemistry · 2022Article
- The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.Cells · 2021Review
- Timeless couples G-quadruplex detection with processing by DDX11 helicase during DNA replication.The EMBO journal · 2020Article
- Molecular Basis for ATP-Hydrolysis-Driven DNA Translocation by the CMG Helicase of the Eukaryotic Replisome.Cell reports · 2019Article
- The DNA Pol ϵ stimulatory activity of Mrc1 is modulated by phosphorylation.Cell cycle (Georgetown, Tex.) · 2018Article
- Checkpoint-Independent Regulation of Origin Firing by Mrc1 through Interaction with Hsk1 Kinase.Molecular and cellular biology · 2017Article
- The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.PloS one · 2015Article
- CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin.Cell cycle (Georgetown, Tex.) · 2015Article
- The human Tim-Tipin complex interacts directly with DNA polymerase epsilon and stimulates its synthetic activity.The Journal of biological chemistry · 2013Article
- Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.Molecular and cellular biology · 2012Article
- Swi1 associates with chromatin through the DDT domain and recruits Swi3 to preserve genomic integrity.PloS one · 2012Article
- Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiation.The Journal of cell biology · 2011Article
- Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.Molecular and cellular biology · 2011Article
- Characterization of functional domains in human Claspin.Cell cycle (Georgetown, Tex.) · 2011Article
- Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates.PloS one · 2011Article
- Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.Cell cycle (Georgetown, Tex.) · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication fork protection complex Swi1-Swi3 and replication checkpoint mediator Mrc1 are required for maintenance of replication fork integrity during the course of DNA replication in the fission yeast Schizosaccharomyces pombe. These proteins play crucial roles in stabilizing stalled forks and activating replication checkpoint signaling pathways. Although they are conserved replication fork components, precise biochemical roles of these proteins are not known. Here we purified Mrc1 and Swi1-Swi3 proteins and show that these proteins bind to DNA independently but synergistically in vitro. Mrc1 binds preferentially to arrested fork or D-loop-like structures, although the affinity is relatively low, whereas the Swi1-Swi3 complex binds to double-stranded DNA with higher affinity. In the presence of a low concentration of Swi1-Swi3, Mrc1 generates a novel ternary complex and binds to various types of DNA with higher affinity. Moreover, purified Mrc1 and Swi1-Swi3 physically interact with each other, and this interaction is lost by mutations in the known DNA binding domain of Mrc1 (K235E,K236E). The interaction is also lost in a mutant form of Swi1 (E662K) that is specifically defective in polar fork arrest at a site called RTS1 and causes sensitivity to genotoxic agents, although the DNA binding affinity of Swi1-Swi3 is not affected by this mutation. As expected, the synergistic effect of the Swi1-Swi3 on DNA binding of Mrc1 is also lost by these mutations affecting the interaction between Mrc1 and Swi1-Swi3. Our results reveal an aspect of molecular interactions that may play an important role in replication pausing and fork stabilization.
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